Neuropeptide expression and morphometric differences in crushed alveolar inferior nerve of rats: Effects of photobiomodulation
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Data
2017-05-01
Autores
Martins, Daniel Oliveira
Santos, Fabio Martinez dos
Ciena, Adriano Polican [UNESP]
Watanabe, Ii-Sei
Britto, Luiz Roberto G. de
Dias Lemos, Jose Benedito
Chacur, Marucia
Título da Revista
ISSN da Revista
Título de Volume
Editor
Springer
Resumo
Inferior alveolar nerve (IAN) injuries may occur during various dental routine procedures, especially in the removal of impacted lower third molars, and nerve recovery in these cases is a great challenge in dentistry. Here, the IAN crush injury model was used to assess the efficacy of photobiomodulation (PBM) in the recovery of the IAN in rats following crushing injury (a partial lesion). Rats were divided into four experimental groups: without any procedure, IAN crush injury, and IAN crush injury with PBM and sham group with PBM. Treatment was started 2 days after surgery, above the site of injury, and was performed every other day, totaling 10 sessions. Rats were irradiated with GaAs Laser (Gallium Arsenide, Laserpulse, Ibramed Brazil) emitting a wavelength of 904 nm, an output power of 70 mWpk, beam spot size at target similar to 0.1 cm(2), a frequency of 9500 Hz, a pulse time 60 ns, and an energy density of 6 J/cm(2). Nerve recovery was investigated by measuring the morphometric data of the IAN using TEM and by the expression of laminin, neurofilaments (NFs), and myelin protein zero (MPZ) using Western blot analysis. We found that IAN-injured rats which received PBM had a significant improvement of IAN morphometry when compared to IAN-injured rats without PBM. In parallel, all MPZ, laminin, and NFs exhibited a decrease after PBM. The results of this study indicate that the correlation between the peripheral nerve ultrastructure and the associated protein expression shows the beneficial effects of PBM.
Descrição
Palavras-chave
Molecular changes, Alveolar inferior nerve, Photobiomodulation, Myelin sheath, Rat
Como citar
Lasers In Medical Science. London: Springer London Ltd, v. 32, n. 4, p. 833-840, 2017.