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dc.contributor.authorTakamiya, Aline Satie [UNESP]
dc.contributor.authorMonteiro, Douglas Roberto [UNESP]
dc.contributor.authorBernabe, Daniel Galera [UNESP]
dc.contributor.authorGorup, Luiz Fernando
dc.contributor.authorCamargo, Emerson Rodrigues
dc.contributor.authorGomes-Filho, Joao Eduardo [UNESP]
dc.contributor.authorPenha Oliveira, Sandra Helena [UNESP]
dc.contributor.authorBarbosa, Debora Barros [UNESP]
dc.date.accessioned2018-11-26T16:40:39Z
dc.date.available2018-11-26T16:40:39Z
dc.date.issued2016-06-01
dc.identifierhttp://dx.doi.org/10.1016/j.joen.2016.03.014
dc.identifier.citationJournal Of Endodontics. New York: Elsevier Science Inc, v. 42, n. 6, p. 953-960, 2016.
dc.identifier.issn0099-2399
dc.identifier.urihttp://hdl.handle.net/11449/161606
dc.description.abstractIntroduction: Silver nanoparticles have been used for different purposes in dentistry, including endodontic treatments. The aim of this study was to determine the cytotoxicity of different types of silver nanoparticles on mouse fibroblast cell line L929 and the reaction of subcutaneous connective tissue of Wistar rats to these nanoparticles. Methods: Silver nanoparticles of an average size of 5 nm were synthesized with ammonia (SNA) or polyvinylpyrrolidone (SNP). L929 was exposed to SNA and SNP (0.1-100 mu g/mL), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and enzyme-linked immunosorbent assays were performed after 6, 24, and 48 hours. Culture medium was used as the control. Sixteen rats received, individually, 3 polyethylene tubes filled with a fibrin sponge embedded in 100 mu L SNA or SNP (1 mu g/mL). A fibrin sponge with no embedding was the control. Tissue reaction was performed qualitatively and quantitatively after 7, 15, 30, and 90 days of implantation in the dorsal connective tissue of Wistar rats. Results: SNA and SNP were cytotoxic to L929 in higher concentrations, with SNA significantly more toxic than SNP. SNA and SNP did not induce significant interleukin-1 beta and interleukin-6 production. The release of stern cell factor by L929 increased 48 hours after the treatment with SNP at 5 mu g/rnL. Histologic examination showed that the inflammatory responses caused by SNA and SNP at 1 mu g/mL were similar to the control in all experimental periods. Conclusions: It was concluded that SNA and SNP were not cytotoxic at 25 mu g/mL or lower concentrations. However, for safe clinical use, further studies establishing others points of its toxicologic profile are recommended.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent953-960
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal Of Endodontics
dc.sourceWeb of Science
dc.subjectBiocompatibility
dc.subjectendodontic materials
dc.subjectinflammation
dc.subjectsilver nanoparticles
dc.subjecttoxicity
dc.titleIn Vitro and In Vivo Toxicity Evaluation of Colloidal Silver Nanoparticles Used in Endodontic Treatmentsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.description.affiliationUniv Estadual Paulista, Aracatuba Dent Sch, Dept Pediat Dent & Publ Hlth Dent, Aracatuba, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Aracatuba Dent Sch, Dept Pathol & Clin Propedeut, Aracatuba, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Aracatuba Dent Sch, Dept Basic Sci, Aracatuba, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Aracatuba Dent Sch, Dept Endodont, Aracatuba, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, Aracatuba, SP, Brazil
dc.description.affiliationUniv Fed Sao Carlos, Dept Chem, Interdisciplinary Lab Electrochem & Ceram, BR-13560 Sao Carlos, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Aracatuba Dent Sch, Dept Pediat Dent & Publ Hlth Dent, Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Aracatuba Dent Sch, Dept Pathol & Clin Propedeut, Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Aracatuba Dent Sch, Dept Basic Sci, Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Aracatuba Dent Sch, Dept Endodont, Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, Aracatuba, SP, Brazil
dc.identifier.doi10.1016/j.joen.2016.03.014
dc.identifier.wosWOS:000377821200019
dc.rights.accessRightsAcesso aberto
dc.description.sponsorshipIdFAPESP: 2013/07296-2
dc.description.sponsorshipIdFAPESP: 2010/05788-7
dc.identifier.fileWOS000377821200019.pdf
unesp.author.orcid0000-0003-2646-8026[4]
dc.relation.ispartofsjr1,585
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