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dc.contributor.authorDe Almeida, Carolina V. [UNESP]
dc.contributor.authorZamame, Jofer A. [UNESP]
dc.contributor.authorRomagnoli, Graziela G. [UNESP]
dc.contributor.authorRodrigues, Cecilia P.
dc.contributor.authorMagalhaes, Marianna B. [UNESP]
dc.contributor.authorAmedei, Amedeo
dc.contributor.authorKaneno, Ramon [UNESP]
dc.date.accessioned2018-11-26T17:34:54Z
dc.date.available2018-11-26T17:34:54Z
dc.date.issued2017-07-01
dc.identifierhttp://dx.doi.org/10.3892/or.2017.5692
dc.identifier.citationOncology Reports. Athens: Spandidos Publ Ltd, v. 38, n. 1, p. 561-568, 2017.
dc.identifier.issn1021-335X
dc.identifier.urihttp://hdl.handle.net/11449/162907
dc.description.abstractNon-cytotoxic concentrations of selected chemotherapeutic agents amplify the antigen presentation capacity of dendritic cells (DCs) and are able to increase the immunogenicity of the colon cancer cell lineage HCT-116, as previously demonstrated by our group. Since this functional alteration was associated with changes in gene expression, we aimed to evaluate whether transcriptional changes of tumor cells can be transferred to DCs, increasing their ability to induce a specific antitumor response. Therefore, HCT-116 cells were treated with two different concentrations of 5-fluorouracil (5-FU), and their total RNA was transfected into human monocyte-derived DC, which function was evaluated through their ability to stimulate the proliferation of normal allogeneic T lymphocytes (MLR), and to generate cytolytic T cells. The transfected DCs demonstrated an increased percentage of CD83(+), HLA-DR+, CD80(+) and CD86(+) cells. These phenotypical changes were followed by functional improvement demonstrated by the increased capacity of these DC to induce allogeneic T cell proliferation and to generate specific anti-HCT-116 cytolytic T cells, as demonstrated by IFN-gamma production following in vitro challenge with tumor cells. Our results allow us to conclude that treatment of tumor cells with a non-toxic concentration of 5-FU induces immunogenic changes that are transferred to DC by transfection of total RNA.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipregional contribution of 'the Programma Attuativo Regionale (Toscana) cofinanziato dal FAS (adesso FSC) - PAR FAS'
dc.format.extent561-568
dc.language.isoeng
dc.publisherSpandidos Publ Ltd
dc.relation.ispartofOncology Reports
dc.sourceWeb of Science
dc.subjectchemoimmunomodulation
dc.subjectcolorectal cancer
dc.subjectdendritic cells
dc.subjectRNA transfection
dc.subjectvaccine
dc.titleTreatment of colon cancer cells with 5-fluorouracil can improve the effectiveness of RNA-transfected antitumor dendritic cell vaccineen
dc.typeArtigo
dcterms.rightsHolderSpandidos Publ Ltd
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniv Florence
dc.contributor.institutionCareggi Univ Hosp AOUC
dc.contributor.institutionMax Planck Inst Immunobiol & Epigenet
dc.description.affiliationSao Paulo State Univ UNESP, Sch Med Botucatu, Dept Pathol, Botucatu, SP, Brazil
dc.description.affiliationUniv Florence, Dept Expt & Clin Med, Largo Brambilla 03, I-50134 Florence, Italy
dc.description.affiliationSao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Microbiol & Immunol, Botucatu, SP, Brazil
dc.description.affiliationCareggi Univ Hosp AOUC, Neuromusculoskeletal Dept Interdisciplinary Inter, I-50134 Florence, Italy
dc.description.affiliationMax Planck Inst Immunobiol & Epigenet, Dept Epigenet, Freiburg, Germany
dc.description.affiliationUnespSao Paulo State Univ UNESP, Sch Med Botucatu, Dept Pathol, Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Microbiol & Immunol, Botucatu, SP, Brazil
dc.identifier.doi10.3892/or.2017.5692
dc.identifier.wosWOS:000404089500064
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdFAPESP: 2009/18331-8
dc.description.sponsorshipIdCNPq: 140541/2012-8
dc.description.sponsorshipIdCNPq: 303952/2010-5
dc.description.sponsorshipIdFAPESP: 2009/16311-0
dc.description.sponsorshipIdFAPESP: 2009/18433-5
dc.description.sponsorshipId: 2012/20494-5
unesp.author.orcid0000-0002-4292-3298[7]
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