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dc.contributor.authorCaneguim, Breno H.
dc.contributor.authorCerri, Paulo Sérgio [UNESP]
dc.contributor.authorSpolidório, Luis Carlos [UNESP]
dc.contributor.authorMiraglia, Sandra M.
dc.contributor.authorCerri, Estela Sasso [UNESP]
dc.date.accessioned2014-05-20T13:46:12Z
dc.date.available2014-05-20T13:46:12Z
dc.date.issued2009-02-25
dc.identifierhttp://dx.doi.org/10.1186/1477-7827-7-19
dc.identifier.citationReproductive Biology and Endocrinology. London: Biomed Central Ltd., v. 7, p. 9, 2009.
dc.identifier.issn1477-7827
dc.identifier.urihttp://hdl.handle.net/11449/16328
dc.description.abstractBackground: Tacrolimus (FK-506) is an immunosuppressant that binds to a specific immunophilin, resulting in the suppression of the cellular immune response during transplant rejection. Except for some alterations in the spermatozoa, testicular morphological alterations have not been described in rats treated with tacrolimus. In the present study, we purpose to evaluate if the treatment with tacrolimus at long term of follow-up interferes in the integrity of the seminiferous tubules.Methods: Rats aging 42-day-old received daily subcutaneous injections of 1 mg/kg/day of tacrolimus during 30 (T-30) and 60 (T-60) days; the rats from control groups (C-30 and C-60) received saline solution. The left testes were fixed in 4% formaldehyde and embedded in glycol methacrylate for morphological and morphometric analyses while right testes were fixed in Bouin's liquid and embedded in paraffin for detection of cell death by the TUNEL method. The epithelial and total tubular areas as well as the stages of the seminiferous epithelium and the number of spermatocytes, spermatids and Sertoli cells (SC) per tubule were obtained.Results: In the treated groups, seminiferous tubules irregularly outlined showed disarranged cellular layers and loss of germ cells probably due to cell death, which was revealed by TUNEL method. In addition to germ cells, structural alterations in the SC and folding of the peritubular tissue were usually observed. The morphometric results revealed significant decrease in the number of SC, spermatocytes, spermatids and significant reduction in the epithelial and total tubular areas.Conclusion: Tacrolimus induces significant histopathological disorders in the seminiferous tubules, resulting in spermatogenic damage and reduction in the number of Sertoli cells. A careful evaluation of the peritubular components will be necessary to clarify if these alterations are related to the effect of FK-506 on the peritubular tissue.en
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent9
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofReproductive Biology and Endocrinology
dc.sourceWeb of Science
dc.titleStructural alterations in the seminiferous tubules of rats treated with immunosuppressor tacrolimusen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
dcterms.rightsHolderBiomed Central Ltd.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUNESP, Dept Morphol, Lab Histol & Embryol, Sch Dent, Araraquara, Brazil
dc.description.affiliationUniv Fed São Paulo, Dept Morphol & Genet, São Paulo, Brazil
dc.description.affiliationUNESP, Dept Physiol & Pathol, Sch Dent, Araraquara, Brazil
dc.description.affiliationUnespUNESP, Dept Morphol, Lab Histol & Embryol, Sch Dent, Araraquara, Brazil
dc.description.affiliationUnespUNESP, Dept Physiol & Pathol, Sch Dent, Araraquara, Brazil
dc.identifier.doi10.1186/1477-7827-7-19
dc.identifier.wosWOS:000264670600001
dc.rights.accessRightsAcesso aberto
dc.description.sponsorshipIdFAPESP: 06/54776-6
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt
dc.identifier.fileWOS000264670600001.pdf
dc.identifier.lattes3278495911207882
dc.identifier.lattes2640929291808415
dc.identifier.lattes4455630076841302
dc.identifier.orcid0000-0001-5756-5828
unesp.author.lattes3278495911207882[2]
unesp.author.lattes2640929291808415
unesp.author.lattes4455630076841302
unesp.author.orcid0000-0002-0592-542X[3]
unesp.author.orcid0000-0001-5649-4749[4]
unesp.author.orcid0000-0001-5756-5828[2]
dc.relation.ispartofjcr2.852
dc.relation.ispartofsjr1,203
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