ULTRASTRUCTURAL CHANGES AND INTEGRITY OF GENOMIC DNA IN GERMINATED SEEDS OF Peltophorum dubium (Spreng.) TAUBERT SUBJECTED TO DRYING
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Data
2017-01-01
Autores
Guimaraes, Cristiane Carvalho
Oliveira, Julio Maia
Rocha Faria, Jose Marcio
Amaral da Silva, Edvaldo Aparecido [UNESP]
Título da Revista
ISSN da Revista
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Editor
Univ Federal Vicosa
Resumo
The aim of this study was to evaluate DNA integrity and to identify ultrastructural changes in the primary root of Peltophorum dubium seeds subjected to drying, since these changes may reflect the germination capacity. Seeds were germinated and were dehydrated when reached 1 mm in length of the primary root, until reaching different moisture contents, being then rehydrated and evaluated in relation to survival. After this procedure, different points of interest (control, 20% and 10% moisture content) were selected to evaluate DNA and cell ultrastructure integrity in order to detect possible cellular changes in primary roots after dehydration. When dried down to 32% moisture, there was no influence on the growth resumption of the germinated seeds, but when they were dehydrated to 20% moisture, the survival was reduced to 67%, reaching nullity in the survival with drying to 8% moisture. By the evaluation of ultrastructural changes in root cells, it was observed that drying to approximately 20% moisture content marked the onset of damages by desiccation, both physiologically and ultrastructurally. Drying to levels close to 10% moisture content was not only responsible for total disorganization and rupture of membranes, but also promoted intracellular collapse. Loss of genomic DNA integrity was observed during drying.
Descrição
Palavras-chave
Desiccation tolerance, Cellular alterations, DNA damage
Como citar
Revista Arvore. Vicosa: Univ Federal Vicosa, v. 41, n. 4, 10 p., 2017.