Synthesis of the Peptide Ac-Wahx-KTTKS and Evaluation of the Ability to Induce In Vitro Collagen Synthesis
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Data
2016-01-01
Autores
Guglielmi, Danila A. S. [UNESP]
Martinelli, Ariane M. [UNESP]
Rissi, Nathalia C. [UNESP]
Cilli, Eduardo M. [UNESP]
Soares, Christiane P. [UNESP]
Chiavacci, Leila A. [UNESP]
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Editor
Bentham Science Publ Ltd
Resumo
In this work, we report the synthesis of a peptide analogue of the KTTKS, termed Ac-Wahx-KTTKS and evaluate its cytotoxicity and role in biosynthesis of collagen for future application in skin aging. The peptide was obtained with purity higher than 97.5%. In the cytotoxicity assay, we observed non-toxic effects for Ac-WAhx-KTTKS at concentrations below 600 mu M for HaCaT and 500 mu M for HepG2 cells, respectively. After 24 and 48 h it was possible to observe significant changes in collagen synthesis in the groups treated with various concentrations of the peptide. In conclusion, the Ac-Wahx-KTTKS peptide increased collagen synthesis in fibroblasts by 80% and it is a promising candidate for improving skin aging.
Descrição
Palavras-chave
Ac-WAhx-KTTKS, antiaging, collagen biosynthesis, cytotoxicity, peptide synthesis, skin functions
Como citar
Protein And Peptide Letters. Sharjah: Bentham Science Publ Ltd, v. 23, n. 6, p. 544-547, 2016.