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dc.contributor.authorGonçalves, J. M. [UNESP]
dc.contributor.authorLima, L. R. [UNESP]
dc.contributor.authorMoraes, M. L.
dc.contributor.authorRibeiro, S. J.L. [UNESP]
dc.date.accessioned2018-12-11T16:42:40Z
dc.date.available2018-12-11T16:42:40Z
dc.date.issued2016-11-01
dc.identifierhttp://dx.doi.org/10.1016/j.msec.2016.05.101
dc.identifier.citationMaterials Science and Engineering C, v. 68, p. 338-342.
dc.identifier.issn0928-4931
dc.identifier.urihttp://hdl.handle.net/11449/168712
dc.description.abstractLayer-by-Layer (LbL) films containing silk fibroin (SF) and the 40 aminoacid-long amyloid-β peptide (Aβ1-40) were prepared with the purpose of developing a new prototype of an electrochemical immunosensor. The film showed a satisfactory growth in quartz substrate and screen-printed carbon electrodes, as observed by UV-vis spectroscopy and cyclic voltammetric, respectively. The peptide immobilized in LbL films in junction with SF shows secondary structure induced, as shown by circular dichroism measurements, favoring the interaction SF/peptide LbL film with the specific antibody. Immunosensor showed a linear response in the presence of the antibody with concentrations from 0 to 10 ng mL- 1 both analyzed by current changes in 0.3 V and voltammogram area. This system can be applied as a new prototype for preliminary diagnosis of Alzheimer's disease.en
dc.format.extent338-342
dc.language.isoeng
dc.relation.ispartofMaterials Science and Engineering C
dc.sourceScopus
dc.subjectAlzheimer's disease
dc.subjectAmyloid-β peptide
dc.subjectImmunosensor
dc.subjectLayer-by-layer
dc.subjectSilk fibroin
dc.titleImmunosensor for diagnosis of Alzheimer disease using amyloid-β 1-40 peptide and silk fibroin thin filmsen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationInstituto de Química Universidade Estadual Paulista Júlio de Mesquita Filho UNESP, Rua Professor Francisco Degni, 55
dc.description.affiliationInstituto de Ciência e Tecnologia Universidade Federal de São Paulo UNIFESP, Rua Talim, 330
dc.description.affiliationUnespInstituto de Química Universidade Estadual Paulista Júlio de Mesquita Filho UNESP, Rua Professor Francisco Degni, 55
dc.identifier.doi10.1016/j.msec.2016.05.101
dc.rights.accessRightsAcesso aberto
dc.identifier.scopus2-s2.0-84973277831
dc.identifier.file2-s2.0-84973277831.pdf
unesp.author.orcid0000-0003-0213-3686[3]
dc.relation.ispartofsjr1,110
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