Bacterial Biosynthesis of Gold Nanoparticles Using Salmonella enterica subsp. enterica serovar Typhi Isolated from Blood and Stool Specimens of Patients
Carregando...
Data
2017-09-01
Autores
Mortazavi, Seyed Mojtabi
Khatami, Mehrdad
Sharifi, Iraj
Heli, Hossein
Kaykavousi, Keyghobad
Sobhani Poor, Mohammad Hossein
Kharazi, Sam
Nobre, Marcos Augusto Lima [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Salmonella is one of the most common causes of gastroenteritis. In this study, a PCR assay was developed for the rapid detection of microbial cultures of Salmonella enterica sub species of enterica serovar Typhi in samples of patients suspected typhoid. The assay was based on duplicate the STY4669-hypothetical protein gene. The gene primers were designed and blast. Then the bacteria strains were employed for the green synthesis of gold nanoparticles (AuNPs), and one of the strains represented ability to extracellular synthesis of gold nanoparticles. The nanoparticles were characterized using UV–visible spectrophotometer, X-ray powder diffraction, Transmission electron microscopy. The synthesized nanoparticles had a maximum absorption in UV–vis spectra at 556 nm, a crystalline structure, and an average size of 42 nm.
Descrição
Palavras-chave
AuNPs, Extracellular synthesis, Gold nanoparticles, Molecular identification, Salmonella enterica
Como citar
Journal of Cluster Science, v. 28, n. 5, p. 2997-3007, 2017.