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dc.contributor.authorBorsanelli, Ana Carolina [UNESP]
dc.contributor.authorGaetti-Jardim Júnior, Elerson [UNESP]
dc.contributor.authorDöbereiner, Jürgen
dc.contributor.authorDutra, Iveraldo S. [UNESP]
dc.date.accessioned2018-12-11T16:57:48Z
dc.date.available2018-12-11T16:57:48Z
dc.date.issued2015-01-01
dc.identifierhttp://dx.doi.org/10.1590/S0100-736X2015000300005
dc.identifier.citationPesquisa Veterinaria Brasileira, v. 35, n. 3, p. 237-240, 2015.
dc.identifier.issn1678-5150
dc.identifier.issn0100-736X
dc.identifier.urihttp://hdl.handle.net/11449/171938
dc.description.abstractPeriodontitis in cattle is an infectious purulent progressive disease associated with strict anaerobic subgingival biofilm and is epidemiologically related to soil management at several locations of Brazil. This study aimed to detect Treponema species in periodontal pockets of cattle with lesions deeper than 5mm in the gingival sulcus of 6 to 24-month-old animals considered periodontally healthy. We used paper cones to collect the materials, after removal of supragingival plaques, and kept frozen (at -80°C) up to DNA extraction and polymerase chain reaction (PCR) using T. amylovorum, T. denticola, T. maltophilum, T. medium and T. vincentii primers. In periodontal pocket, it was possible to identify by PCR directly, the presence of Treponema amylovorum in 73% of animals (19/26), T. denticola in 42.3% (11/26) and T. maltophilum in 54% (14/26). Among the 25 healthy sites, it was possible to identify T. amylovorum in 18 (72%), T. denticola in two (8%) and T. maltophilum in eight (32%). Treponema medium and T. vincentii were not detected over all 51 evaluated samples. The presence of Treponema amylovorum, T. maltophilum and, in particular, the widely recognized T. denticola in subgingival microflora brings an original and potencially important contribution in studies of the bovine periodontitis.en
dc.format.extent237-240
dc.language.isoeng
dc.relation.ispartofPesquisa Veterinaria Brasileira
dc.sourceScopus
dc.subjectBovine periodontitis
dc.subjectPeriodontal disease
dc.subjectSubgingival microflora
dc.subjectTreponema denticola
dc.titleTreponema denticola in microflora of bovine periodontitisen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationFaculdade de Cências Agrárias e Veterinárias, Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/n
dc.description.affiliationDepartamento de Patologia e Propedêutica Clínica, Faculdade de Odontologia de Araçatuba, Unesp, Rua José Bonifácio 1193
dc.description.affiliationDepartamento de Apoio, Produção e Saúde Animal, Faculdade de Medicina Veterinária de Araçatuba, Unesp, Rua Clóvis Pestana 793
dc.description.affiliationUnespFaculdade de Cências Agrárias e Veterinárias, Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/n
dc.description.affiliationUnespDepartamento de Patologia e Propedêutica Clínica, Faculdade de Odontologia de Araçatuba, Unesp, Rua José Bonifácio 1193
dc.description.affiliationUnespDepartamento de Apoio, Produção e Saúde Animal, Faculdade de Medicina Veterinária de Araçatuba, Unesp, Rua Clóvis Pestana 793
dc.identifier.doi10.1590/S0100-736X2015000300005
dc.rights.accessRightsAcesso restrito
dc.identifier.scopus2-s2.0-84937398647
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