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dc.contributor.authorKotake, Bruna G. dos S.
dc.contributor.authorSalzedas, Leda M. P. [UNESP]
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorCalzzani, Ricardo A. J.
dc.contributor.authorSebald, Walter
dc.contributor.authorIssa, João P. M.
dc.date.accessioned2018-12-11T16:58:08Z
dc.date.available2018-12-11T16:58:08Z
dc.date.issued2015-01-01
dc.identifier.citationCurrent Pharmaceutical Design, v. 21, n. 24, p. 3557-3564, 2015.
dc.identifier.issn1873-4286
dc.identifier.issn1381-6128
dc.identifier.urihttp://hdl.handle.net/11449/172011
dc.description.abstractAlcoholism affects bone repair and this study evaluated the recombinant human BMP-2 (rhBMP-2)/collagen sponge association aiming to improve the bone healing process. The aim of this study was to investigate the action of alcoholism and its effect on the repair of bone defects (BD) performed on rat calvaria after the application of rhBMP-2, either pure or combined with a collagen matrix, using radiographic, histological and immunohistochemical methods. Methods: We used 80 rats divided into two groups and these into 4 subgroups, each with a waiting period for sacrifice of four and six weeks after the BD (5mm). The groups were divided into: Veh-X) vehicle+BD, Veh-BMP) water+BD+5μg rhBMP-2, Veh-ACS) water+ BD+absorbable collagen sponge, Veh-BMP/ACS) water+BD+5μg rhBMP-2/absorbable collagen sponge, EtOH-X) ethanol+BD, EtOH-BMP) ethanol+BD+5μgrhBMP-2, EtOH-ACS) ethanol+BD+absorbable collagen sponge, and EtOH-BMP/ACS) ethanol+ BD+5μg of rhBMP-2/ absorbable collagen sponge. Results: Radiographically, it was found that after six weeks, for the groups treated with rhBMP-2, independent of the carrier use and ethanol administration, there was more new bone formation (p<0.05). For immunohistochemical analysis, osteocalcin and bone sialoprotein were found to be predominant in groups treated with rhBMP-2. For quantitative stereology, which aims to calculate the volume of new bone, higher values for the groups treated with rhBMP-2 pure or combined with the carrier were found; but for the groups treated with ethanol, a higher bone formation in the groups treated with rhBMP-2 associated with the carrier in the periods of four and six weeks (p<0.001) was found. Conclusion: It was concluded that the carrier was effective for rhBMP-2 delivery, even in the presence of ethanol.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent3557-3564
dc.language.isoeng
dc.relation.ispartofCurrent Pharmaceutical Design
dc.sourceScopus
dc.subjectAbsorbable collagen sponge
dc.subjectAlcoholism
dc.subjectBone formation
dc.subjectrhBMP-2
dc.titleBone recuperation after rhBMP-2 insertion in alcoholic animals-experimental studyen
dc.typeArtigo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionTheodor-Boveri-Institut für Biowissenschaften, Am Hubland, Würzburg University
dc.description.affiliationDepartment of Morphology, Physiology and Basic Pathology, Universityof Sao Paulo-USP
dc.description.affiliationDepartment of Pathology and Clinical Propedeutics at the School of Dentistry of Araçatuba, São Paulo State University- UNESP
dc.description.affiliationDepartment of Science Basic at the School of Dentistry ofAraçatuba, São Paulo State University- UNESP
dc.description.affiliationTheodor-Boveri-Institut für Biowissenschaften, Am Hubland, Würzburg University
dc.description.affiliationDepartment of Biomechanics, Medicine and Locomotor Apparatus Rehabilitation, School of Medicine at Ribeirao Preto, University of Sao Paulo-USP
dc.description.affiliationUnespDepartment of Pathology and Clinical Propedeutics at the School of Dentistry of Araçatuba, São Paulo State University- UNESP
dc.description.affiliationUnespDepartment of Science Basic at the School of Dentistry ofAraçatuba, São Paulo State University- UNESP
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdCNPq: 470033/2011-8
dc.identifier.scopus2-s2.0-84939832448
dc.relation.ispartofsjr0,883
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