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dc.contributor.authorde Sandre, Lidiane Cristina Gonçalves [UNESP]
dc.contributor.authorBuzollo, Hellen [UNESP]
dc.contributor.authordo Nascimento, Thiago Matias Torres [UNESP]
dc.contributor.authorNeira, Lígia Maria [UNESP]
dc.contributor.authorAbimorad, Eduardo Gianini
dc.contributor.authorJomori, Rosangela Kiyoko
dc.contributor.authorDucatti, Carlos [UNESP]
dc.contributor.authorPortella, Maria Célia [UNESP]
dc.contributor.authorCarneiro, Dalton José [UNESP]
dc.identifier.citationJournal of the World Aquaculture Society, v. 47, n. 1, p. 113-122, 2016.
dc.description.abstractThis study evaluated the application of stable isotopes of carbon as an alternative and more accurate method to determine gastrointestinal transit time (GTT) in fish by comparing it to the inert marker method. The stable isotope method detects alterations of the normal carbon flow in a biological system by analyzing naturally occurring isotopes of carbon, contrary to studies based on conventional techniques that apply external markers to the diet to determine GTT through visual observation of the color change in feces. Therefore, 320 pacu, Piaractus mesopotamicus juveniles were reared in 32 tanks under two different temperatures (25 and 29 C). The pacu juveniles received two different diets, one based on ingredients derived from C3 photosynthetic cycle plants and the other based on C4 plant ingredients, both containing titanium oxide (TiO2) as a marker. After 40 d, the isotopic signature of the diets was changed, and the marker was replaced by chromic oxide (Cr2O3). In the isotopic technique, the feces were analyzed to determine the exchange in the isotopic ratio of carbon δ13C. Both methods found that GTT was faster (nearly 6h) in fish at 29 C when using the C4/C3 feeding strategy and slower in fish at 25 C using the C3/C4 strategy (15h by inert marker and 18h by the isotopic method). In conclusion, GTT determination in pacu juveniles using the stable isotope technique exhibits the same accuracy obtained with the inert marker method at temperatures suitable (nearly 29 C) for the metabolism of these animals.en
dc.relation.ispartofJournal of the World Aquaculture Society
dc.titleNatural Stable Isotopes for Determination of Gastrointestinal Transit Time in Fishen
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInstituto de Pesca/APTA/SAA
dc.contributor.institutionFaculdade Dr Francisco Maeda/FE
dc.description.affiliationUNESP - Univ Estadual Paulista
dc.description.affiliationInstituto de Pesca/APTA/SAA, Rod Washington Luís
dc.description.affiliationLaboratório de Aquicultura Faculdade Dr Francisco Maeda/FE
dc.description.affiliationUNESP - Univ Estadual Paulista Centro de Isótopos Estáveis
dc.description.affiliationUNESP - Univ Estadual Paulista Centro de Aquicultura
dc.description.affiliationUnespUNESP - Univ Estadual Paulista
dc.description.affiliationUnespUNESP - Univ Estadual Paulista Centro de Isótopos Estáveis
dc.description.affiliationUnespUNESP - Univ Estadual Paulista Centro de Aquicultura
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