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dc.contributor.authorMarcos, Caroline Maria [UNESP]
dc.contributor.authorde Oliveira, Haroldo Cesar [UNESP]
dc.contributor.authorda Silva, Julhiany de Fátima [UNESP]
dc.contributor.authorAssato, Patricia Akemi [UNESP]
dc.contributor.authorYamazaki, Daniella Sayuri [UNESP]
dc.contributor.authorda Silva, Rosângela Aparecida Moraes [UNESP]
dc.contributor.authorSantos, Cláudia Tavares [UNESP]
dc.contributor.authorSantos-Filho, Norival Alves [UNESP]
dc.contributor.authorPortuondo, Deivys Leandro [UNESP]
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.contributor.authorFusco-Almeida, Ana Marisa [UNESP]
dc.date.accessioned2018-12-11T17:07:39Z
dc.date.available2018-12-11T17:07:39Z
dc.date.issued2016-11-01
dc.identifierhttp://dx.doi.org/10.1093/femsyr/fow079
dc.identifier.citationFEMS Yeast Research, v. 16, n. 7, 2016.
dc.identifier.issn1567-1364
dc.identifier.issn1567-1356
dc.identifier.urihttp://hdl.handle.net/11449/173769
dc.description.abstractParacoccidioides spp., which are temperature-dependent dimorphic fungi, are responsible for the most prevalent human systemic mycosis in Latin America, the paracoccidioidomycosis. The aim of this study was to characterise the involvement of elongation factor Tu (EF-Tu) in Paracoccidioides brasiliensis-host interaction. Adhesive properties were examined using recombinant PbEF-Tu proteins and the respective polyclonal anti-rPbEF-Tu antibody. Immunogold analysis demonstrated the surface location of EF-Tu in P. brasiliensis. Moreover, PbEF-Tu was found to bind to fibronectin and plasminogen by enzyme-linked immunosorbent assay, and it was determined that the binding to plasminogen is at least partly dependent on lysine residues and ionic interactions. To verify the participation of EF-Tu in the interaction of P. brasiliensis with pneumocytes, we blocked the respective protein with an anti-rPbEF-Tu antibody and evaluated the consequences on the interaction index by flow cytometry. During the interaction, we observed a decrease of 2- and 3-fold at 8 and 24 h, respectively, suggesting the contribution of EF-Tu in fungal adhesion/invasion.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.language.isoeng
dc.relation.ispartofFEMS Yeast Research
dc.sourceScopus
dc.subjectEF-Tu
dc.subjectfibronectin
dc.subjectfungal-host interaction
dc.subjectmoonlighting protein
dc.subjectParacoccidioides brasiliensis
dc.subjectplasminogen
dc.titleIdentification and characterisation of elongation factor Tu, a novel protein involved in Paracoccidioides brasiliensis-host interactionen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationFaculdade de Ciências Farmacêuticas UNESP - Univ Estadual Paulista Departamento de Análises Clínicas Laboratório de Micologia Clínica
dc.description.affiliationInstituto de Química UNESP - Univ Estadual Paulista Departamento de Bioquímica e Tecnologia Química Unidade de Sintese Estrutura e Caracterizacao de Peptideos e Proteinas
dc.description.affiliationFaculdade de Ciências Farmacéuticas UNESP - Univ Estadual Paulista Departamento de Análises Clínicas Laboratório de Imunologia Clínica
dc.description.affiliationUnespFaculdade de Ciências Farmacêuticas UNESP - Univ Estadual Paulista Departamento de Análises Clínicas Laboratório de Micologia Clínica
dc.description.affiliationUnespInstituto de Química UNESP - Univ Estadual Paulista Departamento de Bioquímica e Tecnologia Química Unidade de Sintese Estrutura e Caracterizacao de Peptideos e Proteinas
dc.description.affiliationUnespFaculdade de Ciências Farmacéuticas UNESP - Univ Estadual Paulista Departamento de Análises Clínicas Laboratório de Imunologia Clínica
dc.identifier.doi10.1093/femsyr/fow079
dc.rights.accessRightsAcesso aberto
dc.description.sponsorshipIdFAPESP: 2015/03700-9
dc.identifier.scopus2-s2.0-84994717965
dc.identifier.file2-s2.0-84994717965.pdf
unesp.author.lattes3716273524139678[11]
unesp.author.orcid0000-0002-2115-8988[11]
dc.relation.ispartofsjr1,308
dc.relation.ispartofsjr1,308
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