Optimized medium culture for Acidobacteria subdivision 1 strains
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Data
2016-01-01
Autores
Campanharo, João Carlos [UNESP]
Kielak, Anna Maria
Castellane, Tereza Cristina Luque [UNESP]
Kuramae, Eiko Eurya
Lemos, Eliana Gertrudes de Macedo [UNESP]
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Resumo
Members of subdivision 1 of the phylum Acidobacteria were grown at different pH values in a new medium formulation named PSYL 5, which includes sucrose as a carbon source and other compounds (such as KH2PO4 and MgSO4.7H2O). Growth rate was nearly constant at pH 5.0 and declined at pH 3-4 and 6-7. However, it was found that effects involving good carbon/nitrogen ratios and pH on the growth of the members of Acidobacteria subdivision 1 were significant, and the strongest effect of these conditions was at pH 5.0. In addition, incubation time of 48, 72, 96 and 120 h was shorter than that described previously for members of Acidobacteria subdivision 1 on solid laboratory media.
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Palavras-chave
Acidobacteria, Acidobacteria subdivision 1, Colony growth, Growth in sucrose
Como citar
FEMS Microbiology Letters, v. 363, n. 21, 2016.