Crystallization and preliminary X-ray diffraction analysis of importin-alpha acomplexed with NLS peptidomimetics
Nenhuma Miniatura disponível
Data
2005-06-15
Autores
Fontes, MRM
Teh, T.
Riell, R. D.
Park, S. B.
Standaert, R. E.
Kobe, B.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
Importin-alpha is the nuclear import receptor that recognizes cargo proteins with nuclear localization sequences (NLSs). Tile study of NLS peptidomimetics can provide a better understanding of the requirements for the molecular recognition of cargo proteins by importin-alpha, and potentially engender a large number of applications in medicine. Importin-a was crystallized with a set of six NLS peptidomimetics, and X-ray diffraction data were collected in the range 2.1-2.5 angstrom resolution. Preliminary electron density calculations show that the ligands are present in the crystals. (c) 2005 Elsevier B.V All rights reserved.
Descrição
Palavras-chave
crystallization, X-ray crystallography, importin-alpha, karyopherin-alpha, nuclear localization sequence, NLS peptidomimetic ligand
Como citar
Biochimica Et Biophysica Acta-proteins and Proteomics. Amsterdam: Elsevier B.V., v. 1750, n. 1, p. 9-13, 2005.