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dc.contributor.authorMarchi-Salvador, Daniela P. [UNESP]
dc.contributor.authorCorrea, Luiz C. [UNESP]
dc.contributor.authorMagro, Angelo J. [UNESP]
dc.contributor.authorOliveira, Clayton Z.
dc.contributor.authorSoares, Andreimar M.
dc.contributor.authorFontes, Marcos R. M. [UNESP]
dc.date.accessioned2014-05-20T13:49:34Z
dc.date.available2014-05-20T13:49:34Z
dc.date.issued2008-08-15
dc.identifierhttp://dx.doi.org/10.1002/prot.21980
dc.identifier.citationProteins-structure Function and Bioinformatics. Malden: Wiley-blackwell, v. 72, n. 3, p. 883-891, 2008.
dc.identifier.issn0887-3585
dc.identifier.urihttp://hdl.handle.net/11449/17674
dc.description.abstractCrotoxin B (CB or Cdt PLA(2)) is a basic Asp49-PLA(2) found in the venom of Crotalus durissus terrificus and it is one of the subunits that constitute the crotoxin (Cro). This heterodimeric toxin, main component of the C. d. terrificus venom, is completed by an acidic, nontoxic, and nonenzymatic component (crotoxin A, CA or crotapotin), and it is related to important envenomation effects such as neurological disorders, myotoxicity, and renal failure. Although Cro has been crystallized since 1938, no crystal structure of this toxin or its subunits is currently available. In this work, the authors present the crystal structure of novel tetrameric complex formed by two dimers of crotoxin B isoforms (CB1 and CB2). The results suggest that these assemblies are stable in solution and show that Ser1 and Glu92 of CB1 and CB2, respectively, play an important role in the oligomerization. The tetrameric and dimeric conformations resulting from the association of the isoforms may increase the neurotoxicity of the toxin CB by the creation of new binding sites, which could improve the affinity of the molecular complexes to the presynaptic membrane.en
dc.format.extent883-891
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofProteins: Structure, Function and Bioinformatics
dc.sourceWeb of Science
dc.subjectcrotoxin Ben
dc.subjectAsp49-phospholipase A(2)en
dc.subjectoligomerizationen
dc.subjectneurotoxic activityen
dc.subjectcrystal structureen
dc.subjectCrotalus durissus terrificus venomen
dc.titleInsights into the role of oligomeric state on the biological activities of crotoxin: crystal structure of a tetrameric phospholipase A(2) formed by two isoforms of crotoxin B from Cratalus durissus terrificus venomen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.description.affiliationUNESP, Inst Biociencias, Dept Fis & Biofis, Botucatu, SP, Brazil
dc.description.affiliationUSP, FCFRP, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Fis & Biofis, Botucatu, SP, Brazil
dc.identifier.doi10.1002/prot.21980
dc.identifier.wosWOS:000257689600007
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.author.lattes0059017255172730[3]
unesp.author.orcid0000-0002-4634-6221[6]
unesp.author.orcid0000-0002-4253-6992[3]
dc.relation.ispartofjcr2.274
dc.relation.ispartofsjr1,362
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