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dc.contributor.authorde Barros e Lima Bueno, Rafaela
dc.contributor.authorRam�o, Anelisa
dc.contributor.authorPinheiro, Daniel Guariz [UNESP]
dc.contributor.authorAlves, Cleidson Padua
dc.contributor.authorKannen, Vinicius
dc.contributor.authorJungbluth, Achim A
dc.contributor.authorde Ara�jo, Luiza Ferreira
dc.contributor.authorMuys, Bruna Rodrigues
dc.contributor.authorFonseca, Aline Simoneti
dc.contributor.authorPla�a, Jessica Rodrigues
dc.contributor.authorPanepucci, Rodrigo Alexandre
dc.contributor.authorNeder, Luciano
dc.contributor.authorSaggioro, Fabiano P
dc.contributor.authorMamede, Rui Celso M.
dc.contributor.authorFigueiredo, David Livingstone Alves
dc.contributor.authorSilva, Wilson Ara�jo
dc.identifier.citationTumor Biology, v. 37, n. 11, p. 15087-15096, 2016.
dc.description.abstractLaryngeal squamous cell carcinoma (LSCC) is a very aggressive cancer, considered to be a subtype of the head and neck squamous cell carcinoma (HNSCC). Despite significant advances in the understanding and treatment of cancer, prognosis of patients with LSCC has not improved recently. In the present study, we sought to understand better the genetic mechanisms underlying LSCC development. Thirty-two tumor samples were collected from patients undergoing surgical resection of LSCC. The samples were submitted to whole-genome cDNA microarray analysis aiming to identify genetic targets in LSCC. We also employed bioinformatic approaches to expand our findings using the TCGA database and further performed functional assays, using human HNSCC cell lines, to evaluate viability, cell proliferation, and cell migration after silencing of selected genes. Eight members of the homeobox gene family (HOX) were identified to be overexpressed in LSCC samples when compared to normal larynx tissue. Quantitative RT-PCR analysis validated the overexpression of HOX gene family members in LSCC. Receiver operating characteristic (ROC) statistical method curve showed that the expression level of seven members of HOX gene family can distinguish tumor from nontumor tissue. Correlation analysis of clinical and gene expression data revealed that HOXC8 and HOXD11 genes were associated with the differentiation degree of tumors and regional lymph node metastases, respectively. Additionally, siRNA assays confirmed that HOXC8, HOXD10, and HOXD11 genes might be critical for cell colony proliferation and cell migration. According to our findings, several members of the HOX genes were overexpressed in LSCC samples and seem to be required in biological processes involved in tumor development. This suggests that HOX genes might play a critical role in the physiopathology of LSCC tumors.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.relation.ispartofTumor Biology
dc.subjectCell migration
dc.subjectGene regulation
dc.subjectHOX genes
dc.subjectLarynx squamous cell carcinoma
dc.titleHOX genes: potential candidates for the progression of laryngeal squamous cell carcinomaen
dc.contributor.institutionUniversity of S�o Paulo
dc.contributor.institutionStem Cell and Cell Therapy and Center for Cell Based Therapy
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionMemorial Sloan-Kettering Cancer Center
dc.contributor.institutionUniversity of Centro-Oeste
dc.contributor.institutionCentro Regional de Hemoterapia de Ribeir�o Preto
dc.description.affiliationDepartment of Genetics Ribeir�o Preto Medical School University of S�o Paulo, Avenida Bandeirantes 3900, Monte Alegre
dc.description.affiliationNational Institute of Science and Technology Stem Cell and Cell Therapy and Center for Cell Based Therapy, Rua Tenente Cat�o Roxo, 2501, Monte Alegre
dc.description.affiliationDepartment of Technology College of Agriculture and Veterinary Sciences UNESP
dc.description.affiliationCenter for Integrative Systems Biology CISBi NAP/USP, Rua Cat�o Roxo, 2501, Monte Alegre
dc.description.affiliationDepartment of Pathology Ribeir�o Preto Medical School University of S�o Paulo, Avenida Bandeirantes 3900, Monte Alegre
dc.description.affiliationDepartment of Pathology Memorial Sloan-Kettering Cancer Center, 1275 York Avenue
dc.description.affiliationDepartment of Ophthalmology Otorhinolaryngology and Head and Neck Surgery Ribeir�o Preto Medical School University of S�o Paulo, Avenida Bandeirantes 3900, Monte Alegre
dc.description.affiliationUniversity of Centro-Oeste, Rua Padre Salvador, 875, Santa Cruz
dc.description.affiliationCentro Regional de Hemoterapia de Ribeir�o Preto, Rua Cat�o Roxo, 2501, Monte Alegre
dc.description.affiliationUnespDepartment of Technology College of Agriculture and Veterinary Sciences UNESP
dc.rights.accessRightsAcesso aberto
dc.description.sponsorshipIdCNPq: 140427/2010-4
dc.description.sponsorshipIdFAPESP: 2012/00588-5
dc.description.sponsorshipIdFAPESP: 2013/08135-2
dc.description.sponsorshipIdCNPq: 559809/2009-3
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