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dc.contributor.authorCarvalho, M. A.
dc.contributor.authorCosta, L. M.A.S.
dc.contributor.authorDa Silveira Santos, D. M.E.
dc.contributor.authorDias, D. R.
dc.contributor.authorZied, D. C. [UNESP]
dc.contributor.authorDias, E. S.
dc.date.accessioned2018-12-11T17:29:47Z
dc.date.available2018-12-11T17:29:47Z
dc.date.issued2016-09-01
dc.identifier.citationJournal of Pure and Applied Microbiology, v. 10, n. 3, p. 1683-1691, 2016.
dc.identifier.issn0973-7510
dc.identifier.urihttp://hdl.handle.net/11449/178324
dc.description.abstractThis study was conducted with the objective of evaluating the growth of six strains of Lentinula edodes (LE1, LE2, LE3, LE4, LE5 and LE6) in five culture media and characterizes strains for enzyme production. The culture medium that provided the best mycelial growth of the strains was used for semi-quantitative tests of ligninase and cellulase activity. Samples for enzyme assays and determination of total proteins were collected at 7, 14, 21 and 28 days of incubation to building an enzymatic curve. The strain LE5 is the most aggressive, with higher mycelial growth rate and mycelial mass production in the basic medium added of 0.01% yeast extract. The addition of biotin and thiamine to the culture medium is not sufficient to substitute yeast extract. The performance of the strains with respect to the production of cellulase and ligninase was different. The LE6 strain excelled in the production of laccase, manganese peroxidase and β-glucosidase with 28 days of incubation and endo-β-1,4-glucanase with 14 days of incubation, while strain LE5 excelled in the production of exo-β-1,4-glucanase with 7 days of incubation.en
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent1683-1691
dc.language.isoeng
dc.relation.ispartofJournal of Pure and Applied Microbiology
dc.sourceScopus
dc.subjectBiotin
dc.subjectCellulase
dc.subjectLigninase
dc.subjectShiitake
dc.subjectThiamine
dc.titleLigninase and cellulase activity of Lentinula edodes (Berk.) pegler strains in different culture mediaen
dc.typeArtigo
dc.contributor.institutionUniversidade Federal de Lavras (UFLA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationDepartment of Biology UFLA University of Lavras Campus, PO Box 3037
dc.description.affiliationDepartment of Food Science UFLA University of Lavras, PO Box 3037
dc.description.affiliationSao Paulo State University (UNESP) School of Agricultural Sciences and Technology (FCAT)
dc.description.affiliationUnespSao Paulo State University (UNESP) School of Agricultural Sciences and Technology (FCAT)
dc.rights.accessRightsAcesso restrito
dc.identifier.scopus2-s2.0-84990062607
dc.relation.ispartofsjr0,123
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