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dc.contributor.authorCurbete, Mariane Machado [UNESP]
dc.contributor.authorSalgado, Hérida Regina Nunes [UNESP]
dc.date.accessioned2018-12-11T17:30:30Z
dc.date.available2018-12-11T17:30:30Z
dc.date.issued2016-07-01
dc.identifierhttp://dx.doi.org/10.1590/s1984-82502016000300011
dc.identifier.citationBrazilian Journal of Pharmaceutical Sciences, v. 52, n. 3, p. 447-457, 2016.
dc.identifier.issn2175-9790
dc.identifier.issn1984-8250
dc.identifier.urihttp://hdl.handle.net/11449/178472
dc.description.abstractFusidic acid is an antibiotic steroid indicated for the treatment of infections caused by the genus Staphylococcus, including methicillin resistant Staphylococcus aureus strains, and other Gram-positive bacteria. In the present study, a stability-indicating reversed-phase liquid chromatography (RP-LC) method was developed and validated for the determination of fusidic acid in dermatological cream as an alternative to existing methods. Analyses were performed using a C18 column and guard column at room temperature, eluting with an isocratic mobile phase of acetonitrile and water (72: 28, v/v), adjusted to pH 3.5 with acetic acid, pumped at a flow rate of 1.0 mL min-1, detection at 210 nm and 20 µL of injection volume. The forced degradation study was conducted under acidic, alkaline, neutral, photolytic, and oxidative stress conditions. The method was validated according to ICH and FDA guidelines; it was linear, precise, accurate, selective, and robust over concentrations of 5-95 µg mL-1, with detection and quantification limits of 0.43 and 1.31 µg mL-1, respectively. Therefore, we conclude that this method is suitable for quantifying fusidic acid in pharmaceutical dermatological creams and determining its stability, representing a more economical and practical alternative for routine analysis in quality control.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent447-457
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Pharmaceutical Sciences
dc.sourceScopus
dc.subjectChromatography/reversed-phase
dc.subjectFusidic acid/stability-indicating/quality control/validation
dc.titleStability-indicating RP-LC method for quantification of fusidic acid in creamen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationDepartment of Pharmaceuticals School of Pharmaceutical Sciences UNESP - Univ Estadual Paulista, Campus de Araraquara
dc.description.affiliationUnespDepartment of Pharmaceuticals School of Pharmaceutical Sciences UNESP - Univ Estadual Paulista, Campus de Araraquara
dc.identifier.doi10.1590/s1984-82502016000300011
dc.identifier.scieloS1984-82502016000300447
dc.rights.accessRightsAcesso aberto
dc.description.sponsorshipIdFAPESP: 2013/02861-3
dc.identifier.scopus2-s2.0-85005987338
dc.identifier.fileS1984-82502016000300447.pdf
dc.relation.ispartofsjr0,214
dc.relation.ispartofsjr0,214
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