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dc.contributor.authorde Moraes, Carolina Nogueira [UNESP]
dc.contributor.authorMaia, Leandro [UNESP]
dc.contributor.authorde Oliveira, Eliandre
dc.contributor.authorde Paula Freitas Dell'Aqua, Camila [UNESP]
dc.contributor.authorChapwanya, Aspinas
dc.contributor.authorda Cruz Landim-Alvarenga, Fernanda [UNESP]
dc.contributor.authorOba, Eunice [UNESP]
dc.date.accessioned2018-12-11T17:32:17Z
dc.date.available2018-12-11T17:32:17Z
dc.date.issued2017-05-01
dc.identifierhttp://dx.doi.org/10.1016/j.vetimm.2017.03.007
dc.identifier.citationVeterinary Immunology and Immunopathology, v. 187, p. 42-47.
dc.identifier.issn1873-2534
dc.identifier.issn0165-2427
dc.identifier.urihttp://hdl.handle.net/11449/178830
dc.description.abstractThe use of the conditioned medium (CM) for diseases treatment is based on its enrichment with biomolecules with therapeutic properties and themselves have a beneficial effect. Secretome of bovine endometrial mesenchymal progenitor/stem cells (eMSCs) using a proteomics approach is until now unknown. This work aimed to evaluate the secretome of bovine eMSCs-CM challenged or not with lipopolysaccharide (LPS). For this, eMSCs characterized were challenged (TG) or not (CG). The CM was collected 12 h after stimulation and submitted to mass spectrometry analysis. The classification of identified proteins was done by PANTHER according to biological processes, molecular function, cellular component and protein class. 397 protein groups were identified in TG and 302 in CG. We observed positive enrichment for antibacterial response proteins, macrophage activation function, receptor-mediated endocytosis, hydrolase activity, inhibitory enzyme in TG, and for activity structural molecule and intermediate filament cytoskeleton in the CG. Our experimental model shows that eMSCs respond to LPS in the concentration used and can be used to study immune-inflammatory response, besides of the secretion of proteins mainly related to tissue remodeling, immune response and angiogenesis which is an interesting feature for use in cell therapy.en
dc.format.extent42-47
dc.language.isoeng
dc.relation.ispartofVeterinary Immunology and Immunopathology
dc.sourceScopus
dc.subjectCow
dc.subjectEndometrial mesenchymal cells
dc.subjectnanoLC–MS/MS
dc.subjectProtein
dc.subjectUterus
dc.titleShotgun proteomic analysis of the secretome of bovine endometrial mesenchymal progenitor/stem cells challenged or not with bacterial lipopolysaccharideen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionParc Cientific de Barcelona
dc.contributor.institutionRoss University School of Veterinary Medicine
dc.description.affiliationDepartment of Animal Reproduction and Radiology UNESP – São Paulo State University
dc.description.affiliationProteomics Platform Parc Cientific de Barcelona
dc.description.affiliationRoss University School of Veterinary Medicine Department of Clinical Sciences
dc.description.affiliationUnespDepartment of Animal Reproduction and Radiology UNESP – São Paulo State University
dc.identifier.doi10.1016/j.vetimm.2017.03.007
dc.rights.accessRightsAcesso aberto
dc.identifier.scopus2-s2.0-85018469510
dc.identifier.file2-s2.0-85018469510.pdf
dc.relation.ispartofsjr0,680
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