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dc.contributor.authorSegabinazzi, Lorenzo G. [UNESP]
dc.contributor.authorFriso, Aime M. [UNESP]
dc.contributor.authorCorreal, Sebastian B.
dc.contributor.authorCrespilho, André M.
dc.contributor.authorDell'Aqua, José Antonio [UNESP]
dc.contributor.authorMiró, Jordi
dc.contributor.authorPapa, Frederico O. [UNESP]
dc.contributor.authorAlvarenga, Marco Antonio [UNESP]
dc.date.accessioned2018-12-11T17:33:44Z
dc.date.available2018-12-11T17:33:44Z
dc.date.issued2017-12-01
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2017.08.007
dc.identifier.citationTheriogenology, v. 104, p. 120-126.
dc.identifier.issn0093-691X
dc.identifier.urihttp://hdl.handle.net/11449/179104
dc.description.abstractPersistent mating-induced endometritis (PMIE) results in decreased fertility in horses, thereby causing a significant impact in the horse market. Platelet-rich plasma (PRP), a modulator of the inflammatory response, has been largely used in veterinary medicine. Here, we investigated the effects of PRP on uterine inflammation, conception rate, endometrial polymorphonuclear neutrophil (PMN) migration, and COX-2 protein levels in the endometrial tissue. Thirteen PMIE-susceptible mares were used for artificial insemination (AI). The mares were inseminated with fresh semen in three consecutive cycles in a cross-over study design. The following cycle classifications were used: control cycle, no pharmacological interference; pre-AI, 20 mL of PRP was infused 24 h before AI; and post-AI, 20 mL of PRP was infused four h after AI. Follicular dynamics were monitored daily by transrectal ultrasound. When a follicle larger than 35 mm was detected, ovulation was induced with deslorelin acetate (1 mg, im). AI was performed 24 h after ovulation induction. Intrauterine fluid (FLU) was evaluated by ultrasonography before and 24 h after AI. PMNs in uterine cytology (CYT) and biopsy (HIS) were also observed before and 24 h after AI. Pregnancy was determined within 14 days after ovulation. Number of COX-2 positive cells was evaluated by immunohistochemistry. Both PRP treatments resulted in a decrease of PMNs in the CYT after breeding when compared to controls. FLU did not differ between cycles; however, the conception rates were significantly higher in the PRP mares. Mares positive for endometritis decreased in both treatment groups, and a more intense positive COX-2 labeling was observed in the control group when compared to the two treatment groups. In conclusion, PRP beneficially reduces inflammatory response in PMIE mares independent of when treatments were administered, thus increasing chances of successful pregnancy.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent120-126
dc.language.isoeng
dc.relation.ispartofTheriogenology
dc.sourceScopus
dc.subjectEmbryo transfer
dc.subjectEndometritis
dc.subjectEquine
dc.subjectPlatelet-rich plasma
dc.titleUterine clinical findings, fertility rate, leucocyte migration, and COX-2 protein levels in the endometrial tissue of susceptible mares treated with platelet-rich plasma before and after AIen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUNISA
dc.contributor.institutionSeverino Sombra University
dc.contributor.institutionAutonomous University of Barcelona
dc.description.affiliationDepartment of Animal Reproduction and Veterinary Radiology São Paulo State University – UNESP
dc.description.affiliationSanto Amaro University UNISA
dc.description.affiliationSeverino Sombra University, Vassouras
dc.description.affiliationEquine Reproduction Service Department of Animal Medicine and Surgery Faculty of Veterinary Medicine Autonomous University of Barcelona
dc.description.affiliationUnespDepartment of Animal Reproduction and Veterinary Radiology São Paulo State University – UNESP
dc.identifier.doi10.1016/j.theriogenology.2017.08.007
dc.rights.accessRightsAcesso aberto
dc.description.sponsorshipIdFAPESP: 2015/00150-8
dc.identifier.scopus2-s2.0-85027502459
dc.identifier.file2-s2.0-85027502459.pdf
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