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dc.contributor.authorBoni, Fernanda Isadora [UNESP]
dc.contributor.authorAlmeida, Andreia
dc.contributor.authorLechanteur, Anna
dc.contributor.authorSarmento, Bruno
dc.contributor.authorCury, Beatriz Stringhetti Ferreira [UNESP]
dc.contributor.authorGremião, Maria Palmira Daflon [UNESP]
dc.date.accessioned2018-12-11T17:34:21Z
dc.date.available2018-12-11T17:34:21Z
dc.date.issued2018-01-01
dc.identifierhttp://dx.doi.org/10.1016/j.ejps.2017.09.042
dc.identifier.citationEuropean Journal of Pharmaceutical Sciences, v. 111, p. 73-82.
dc.identifier.issn1879-0720
dc.identifier.issn0928-0987
dc.identifier.urihttp://hdl.handle.net/11449/179243
dc.description.abstractNanostructured polyelectrolytes complexes (nano PECs) loaded with methotrexate (MTX) were obtained by the polyelectrolyte complexation of chitosan (CS) and hyaluronic acid (HA), further incorporating hypromellose phthalate (HP). The mean diameter of nano PECs ranged from 325 to 458 nm, with a narrow size distribution. Zeta potential was close to + 30 mV, decreasing to + 21 mV after the incorporation of HP, a range of values that favour the physical stability of system as the interaction with cationic biological membranes. The electrostatic interactions between the different components were indicated by the FTIR data. The mucoadhesiveness of nano PECs was demonstrated and MTX and HP influenced this property. The cell viability assays showed the biosafety of the isolated polymers and nano PECs in intestinal HT29-MTX and Caco-2 cell lines at 4 h of test. The permeability values of MTX loaded in CS/HA nano PECs were 7.6 and 4-fold higher than those of CS/HA/HP nano PECS and free drug, respectively, in the Caco-2 monoculture. In mucus secreting co-culture cell model these values were 3 and 6.5 fold, respectively. Such features indicate that nano PECs developed in this work can be promising carriers for MTX in the treatment of local or systemic diseases.en
dc.description.sponsorshipEuropean Regional Development Fund
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação para a Ciência e a Tecnologia
dc.format.extent73-82
dc.language.isoeng
dc.relation.ispartofEuropean Journal of Pharmaceutical Sciences
dc.sourceScopus
dc.subjectCaco-2 monoculture
dc.subjectChitosan
dc.subjectIntestinal permeability
dc.subjectMethotrexate
dc.subjectMucoadhesive
dc.subjectPolyelectrolytes complexes
dc.subjectTriple co-culture cell model
dc.titleMucoadhesive nanostructured polyelectrolytes complexes modulate the intestinal permeability of methotrexateen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade do Porto
dc.contributor.institutionCESPU - Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde
dc.contributor.institutionUniversite of Liège
dc.description.affiliationUNESP - São Paulo State University School of Pharmaceutical Sciences, Rod. Araraquara-Jaú, Km 01
dc.description.affiliationi3S - Instituto de Investigação e Inovação em Saúde Universidade do Porto, Rua Alfredo Allen, 208
dc.description.affiliationINEB - Instituto de Engenharia Biomédica Universidade do Porto, Rua Alfredo Allen, 208
dc.description.affiliationCESPU - Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Rua Central de Gandra 1317
dc.description.affiliationLaboratory of Pharmaceutical Technology and Biopharmacy CIRM Universite of Liège
dc.description.affiliationUnespUNESP - São Paulo State University School of Pharmaceutical Sciences, Rod. Araraquara-Jaú, Km 01
dc.identifier.doi10.1016/j.ejps.2017.09.042
dc.rights.accessRightsAcesso aberto
dc.description.sponsorshipIdEuropean Regional Development Fund: NORTE-01-0145-FEDER-000012
dc.description.sponsorshipIdFundação para a Ciência e a Tecnologia: POCI-01-0145-FEDER-007274
dc.identifier.scopus2-s2.0-85030159782
dc.identifier.file2-s2.0-85030159782.pdf
dc.identifier.lattes9129780536724256
unesp.author.lattes9129780536724256
dc.relation.ispartofsjr1,016
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