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dc.contributor.authorSogayar, M. C.
dc.contributor.authorCamargo, A. A.
dc.contributor.authorBettoni, F.
dc.contributor.authorCarraro, D. M.
dc.contributor.authorPires, L. C.
dc.contributor.authorParmigiani, M. B.
dc.contributor.authorFerreira, E. N.
dc.contributor.authorMoreira, E. S.
dc.contributor.authorLatorre, MRDO
dc.contributor.authorSimpson, AJG
dc.contributor.authorCruz, L. O.
dc.contributor.authorDegaki, T. L.
dc.contributor.authorFesta, F.
dc.contributor.authorMassirer, K. B.
dc.contributor.authorCamargo, F.
dc.contributor.authorCamargo, L. P.
dc.contributor.authorCunha, MAV
dc.contributor.authorDe Souza, S. J.
dc.contributor.authorFaria, M.
dc.contributor.authorGiuliatti, S.
dc.contributor.authorKopp
dc.contributor.authorOliviera, PSL
dc.contributor.authorPaiva, P. B.
dc.contributor.authorPereira, A. A.
dc.contributor.authorPinheiro, D. G.
dc.contributor.authorPuga, R. D.
dc.contributor.authorSouza, JES
dc.contributor.authorAlbuquerque, D. M.
dc.contributor.authorAndrade, LEC
dc.contributor.authorBaia, G. S.
dc.contributor.authorBriones, MRS
dc.contributor.authorCavaleiro-Luna, AMS
dc.contributor.authorCerutti, J. M.
dc.contributor.authorCosta, F. F.
dc.contributor.authorConstanzi-Strauss, E.
dc.contributor.authorEspreafico, E. M.
dc.contributor.authorFerrasi, A. C.
dc.contributor.authorFerro, E. S.
dc.contributor.authorFortes, MAHZ
dc.contributor.authorFurchi, JRF
dc.contributor.authorGianella-Neto, D.
dc.contributor.authorGoldman, G. H.
dc.contributor.authorGoldman, MHS
dc.contributor.authorGruber, A.
dc.contributor.authorGuimaraes, G. S.
dc.contributor.authorHackel, C.
dc.contributor.authorHenrique-Silva, F.
dc.contributor.authorKimura, E. T.
dc.contributor.authorLeoni, S. G.
dc.contributor.authorMacedo, C.
dc.contributor.authorMalnic, B.
dc.contributor.authorManzini, C. V.
dc.contributor.authorMarie, SKN
dc.contributor.authorMartinez-Rossi, N. M.
dc.contributor.authorMenossi, M.
dc.contributor.authorMiracca, E. C.
dc.contributor.authorNagai, M. A.
dc.contributor.authorNobrega, F. G.
dc.contributor.authorNobrega, M. P.
dc.contributor.authorOba-Shinjo, S. M.
dc.contributor.authorOliviera, M. K.
dc.contributor.authorOrabona, G. M.
dc.contributor.authorOtsuke, A. Y.
dc.contributor.authorPaco-Larson, M. L.
dc.contributor.authorPaixao, BMC
dc.contributor.authorPandolfi, JRC
dc.contributor.authorPardini, MIMC
dc.contributor.authorPassos-Bueno, M. R.
dc.contributor.authorPassos, GAS
dc.contributor.authorPesquero, J. B.
dc.contributor.authorPessoa, J. G.
dc.contributor.authorRahal, Paula [UNESP]
dc.contributor.authorRainho, C. A.
dc.contributor.authorReis, C. P.
dc.contributor.authorRicca, T. I.
dc.contributor.authorRodriguez, V
dc.contributor.authorRogatto, Silvia Regina [UNESP]
dc.contributor.authorRomano, C. M.
dc.contributor.authorRomeiro, J. G.
dc.contributor.authorRossi, A.
dc.contributor.authorSa, R. G.
dc.contributor.authorSales, M. M.
dc.contributor.authorSantAnna, S. C.
dc.contributor.authorSantarosa, P. L.
dc.contributor.authorSegato, F.
dc.contributor.authorSilva, W. A.
dc.contributor.authorSilva, IDCG
dc.contributor.authorSilva, N. P.
dc.contributor.authorSoares-Costa, A.
dc.contributor.authorSonati, M. F.
dc.contributor.authorStrauss, B. E.
dc.contributor.authorTajara, E. H.
dc.contributor.authorValentini, Sandro Roberto [UNESP]
dc.contributor.authorVillanova, F. E.
dc.contributor.authorWard, L. S.
dc.contributor.authorZanette, D. L.
dc.date.accessioned2014-05-20T13:50:12Z
dc.date.available2014-05-20T13:50:12Z
dc.date.issued2004-07-01
dc.identifierhttp://dx.doi.org/10.1101/gr.2111304
dc.identifier.citationGenome Research. Cold Spring Harbor: Cold Spring Harbor Lab Press, Publications Dept, v. 14, n. 7, p. 1413-1423, 2004.
dc.identifier.issn1088-9051
dc.identifier.urihttp://hdl.handle.net/11449/17929
dc.description.abstractWe report the results of a transcript finishing initiative, undertaken for the purpose of identifying and characterizing novel human transcripts, in which RT-PCR was used to bridge gaps between paired EST Clusters, mapped against the genomic sequence. Each pair of EST Clusters selected for experimental validation was designated a transcript finishing unit (TFU). A total of 489 TFUs were selected for validation, and an overall efficiency of 43.1% was achieved. We generated a total of 59,975 bp of transcribed sequences organized into 432 exons, contributing to the definition of the structure of 211 human transcripts. The structure of several transcripts reported here was confirmed during the course of this project, through the generation of their corresponding full-length cDNA sequences. Nevertheless, for 21% of the validated TFUs, a full-length cDNA sequence is not yet available in public databases, and the structure of 69.2% of these TFUs was not correctly predicted by computer programs. The TF strategy provides a significant contribution to the definition of the complete catalog of human genes and transcripts, because it appears to be particularly useful for identification of low abundance transcripts expressed in a restricted Set of tissues as well as for the delineation of gene boundaries and alternatively spliced isoforms.en
dc.format.extent1413-1423
dc.language.isoeng
dc.publisherCold Spring Harbor Lab Press, Publications Dept
dc.relation.ispartofGenome Research
dc.sourceWeb of Science
dc.titleA transcript finishing initiative for closing gaps in the human transcriptomeen
dc.typeArtigo
dcterms.licensehttp://genome.cshlp.org/site/misc/open_access.xhtml
dcterms.rightsHolderCold Spring Harbor Lab Press, Publications Dept
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionLudwig Inst Canc Res
dc.contributor.institutionUniv Ribeirao Preto
dc.contributor.institutionInst Ludwig
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniv Vale Paraiba
dc.description.affiliationUniv São Paulo, Inst Quim, BR-05513970 São Paulo, Brazil
dc.description.affiliationLudwig Inst Canc Res, BR-01509010 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Epidemiol, Sch Publ Hlth, BR-01246904 São Paulo, SP, Brazil
dc.description.affiliationUniv Ribeirao Preto, Dept Quim Informat Bioinformat, BR-14096380 Ribeirao Preto, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Centro Terapia Celular, Hemocentro, BR-14051140 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Dept Clin Med, BR-14051140 Ribeirao Preto, SP, Brazil
dc.description.affiliationInst Ludwig, Lab Biol Computacional, BR-01509010 São Paulo, SP, Brazil
dc.description.affiliationUniv São Paulo, Lab Genet Cardiol Mol, Inst Coracao, BR-05403000 São Paulo, SP, Brazil
dc.description.affiliationUniv Fed São Paulo, Bioinform Lab, Hlth Informatics Dept, BR-04039032 São Paulo, Brazil
dc.description.affiliationUniv Estad Campinas, Dept Clin Med, Fac Ciências Med, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Fed São Paulo, Rheumatol Div, BR-04113001 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Histol Embriol, Inst Ciências Biomed, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniv Fed São Paulo, Dept Microbiol Immunol Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Lab Cellular Mol Endocrinol, Sch Med, BR-01246903 São Paulo, Brazil
dc.description.affiliationUniv Fed São Paulo, Dept Med, Disciplina Endocrinol, Lab Endocrinol Mol, BR-04039002 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Ciências Biomed, Lab Transferencia Genica, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Dept Biol Celular Mol Bioagentes Patogenicas, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Lab Biol Mol, Hemocentro, Fac Med, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUniv Fed Sao Carlos, Dept Genet Evol, BR-13565905 Sao Carlos, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Ciências Farmaceuticas Ribeirao Preto, BR-14040903 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Filosofia Ciências Letras Ribeirao Preto, Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Patol, Fac Med Vet Zootecnia, BR-05508000 São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Genet Med, Fac Ciências Med, BR-13081970 Campinas, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14040900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Bioquim, Inst Quim, BR-05599970 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Neurol, Fac Med, BR-01246903 São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Lab Genoma Funcional, Centro Biol Mol Engenharia Genet, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Genet Evol, Inst Biol, Campinas, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Radiol, Disciplina Oncol, Fac Med, BR-01246903 São Paulo, Brazil
dc.description.affiliationUniv Vale Paraiba, Inst Pesquisa Desenvolvimento, BR-12244000 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Biociencias, Centro Estudios Genoma Humano, Dept Biol, BR-055508900 São Paulo, Brazil
dc.description.affiliationUniv Fed São Paulo, Lab Mol Gynecol, Dept Gynecol, BR-04039001 São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, Sch Pharmacol, Dept Biol Sci, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUniv São Paulo, Discipl Genet, Fac Odontol, BR-14040900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Fed São Paulo, Dept Biofisica, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Biol, Inst Biociencias Letras Ciências Exatas, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Genet, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Bioquim Imunol, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Lab Genet Mol Cancer, Dept Clin Med, Fac Ciências Med, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Patol Clin, Fac Ciências Med, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv São Paulo, Setor Vetores Virals, Lab Cardiol Mol, Inst Coracao, BR-15403000 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Lab Biol Mol, Hemocentro, Fac Med, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Sch Pharmacol, Dept Biol Sci, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Biol, Inst Biociencias Letras Ciências Exatas, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Genet, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
dc.identifier.doi10.1101/gr.2111304
dc.identifier.wosWOS:000222434200023
dc.rights.accessRightsAcesso aberto
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
dc.identifier.fileWOS000222434200023.pdf
dc.identifier.lattes7991082362671212
dc.identifier.lattes3587895085226224
dc.identifier.orcid0000-0001-9200-5391
unesp.author.lattes7991082362671212
unesp.author.lattes3587895085226224[37]
unesp.author.orcid0000-0001-9200-5391[37]
dc.relation.ispartofjcr10.101
dc.relation.ispartofsjr12,367
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