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dc.contributor.authorOkino-Delgado, Clarissa Hamaio [UNESP]
dc.contributor.authorDo Prado, Débora Zanoni [UNESP]
dc.contributor.authorFacanali, Roselaine
dc.contributor.authorMarques, Márcia Mayo Ortiz
dc.contributor.authorNascimento, Augusto Santana [UNESP]
dc.contributor.authorFernandes, Célio Junior da Costa [UNESP]
dc.contributor.authorZambuzzi, William Fernando [UNESP]
dc.contributor.authorFleuri, Luciana Francisco [UNESP]
dc.date.accessioned2018-12-11T17:34:38Z
dc.date.available2018-12-11T17:34:38Z
dc.date.issued2017-10-01
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0186246
dc.identifier.citationPLoS ONE, v. 12, n. 10, 2017.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11449/179305
dc.description.abstractCooking oil waste leads to well-known environmental impacts and its bioremediation by lipase-based enzymatic activity can minimize the high cytotoxic potential. In addition, they are among the biocatalysts most commercialized worldwide due to the versatility of reactions and substrates. However, although lipases are able to process cooking oil wastes, the products generated from this process do not necessarily become less toxic. Thus, the aim of the current study is to analyze the bioremediation of lipase-catalyzed cooking oil wastes, as well as their effect on the cytotoxicity of both the oil and its waste before and after enzymatic treatment. Thus, assessed the post-frying modification in soybean oil and in its waste, which was caused by hydrolysis reaction catalyzed by commercial and home-made lipases. The presence of lipases in the extracts obtained from orange wastes was identified by zymography. The profile of the fatty acid esters formed after these reactions was detected and quantified through gas chromatography and fatty acids profile compared through multivariate statistical analyses. Finally, the soybean oil and its waste, with and without enzymatic treatment, were assessed for toxicity in cytotoxicity assays conducted in vitro using fibroblast cell culture. The soybean oil wastes treated with core and frit lipases through transesterification reaction were less toxic than the untreated oils, thus confirming that cooking oil wastes can be bioremediated using orange lipases.en
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.sourceScopus
dc.titleBioremediation of cooking oil waste using lipases from wastesen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionCEP
dc.description.affiliationChemistry and Biochemistry Department Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationAgronomic Institute (IAC) CEP
dc.description.affiliationUnespChemistry and Biochemistry Department Institute of Biosciences São Paulo State University (UNESP)
dc.identifier.doi10.1371/journal.pone.0186246
dc.rights.accessRightsAcesso aberto
dc.description.sponsorshipIdFAPESP: 2014/ 22689-3
dc.description.sponsorshipIdFAPESP: 2014/10962-7
dc.description.sponsorshipIdFAPESP: 2015/01753-8
dc.identifier.scopus2-s2.0-85032475800
dc.identifier.file2-s2.0-85032475800.pdf
dc.relation.ispartofsjr1,164
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