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dc.contributor.authorKogawa, Ana Carolina [UNESP]
dc.contributor.authorMendonça, Jacqueline Nakau
dc.contributor.authorLopes, Norberto Peporine
dc.contributor.authorSalgado, Hérida Regina Nunes [UNESP]
dc.date.accessioned2018-12-11T17:34:44Z
dc.date.available2018-12-11T17:34:44Z
dc.date.issued2017-11-01
dc.identifierhttp://dx.doi.org/10.2174/1573412912666160801103712
dc.identifier.citationCurrent Pharmaceutical Analysis, v. 13, n. 6, p. 520-524, 2017.
dc.identifier.issn1875-676X
dc.identifier.issn1573-4129
dc.identifier.urihttp://hdl.handle.net/11449/179330
dc.description.abstractIntroduction: Rifaximin is an oral antimicrobial, intestine-selective and non systemic with adverse effects compared to placebo. Materials and Methods: Rifaximin, until then, does not have standardized analytical methods in most official compendia. Fast, economic, environmentally friendly and selective method indicative of stability by thin chromatographic (TC) for the determination of rifaxmin and its degradation products was designed. The TC method used silica gel as stationary phase and ethyl acetate: ethyl alcohol, 90:10 (v/v), as mobile phase to achieve spots for rifaximin (Rf=0.62) and its degradation products basic (Rf=0.33, 0.49, 0.56), acidic (Rf=0.47, 0.54), oxidative (Rf=0.28) and neutral (Rf=0.32). The plates were visualized in chamber UV at 254 nm. Simultaneously, analysis of high performance liquid chromatography (HPLC) was performed using Eclipse Plus C18 (150 mm x 4.6 mm) column and purified water + 0.1 % glacial acetic acid and ethanol in the ratio 52:48 (v/v) as mobile phase at 290 nm. The objective was to verify the interchangeability of TLC and HPLC methods in obtaining the degradation products of rifaximin tablets. In HPLC, as in TC was observed the rifaximin (RT=5.5) and its degradation products acidic, basic (RT =4.2 and 4.7), oxidative (RT =3.6 min) and neutral (RT =4.7 min). Conclusion: These techniques can be used as methods indicative of stability because they identify the rifaximin and its degradation products. Therefore, they can be effectively applied in quality control of rifaximin in tablets. The study of alternative methods should be encouraged by reducing costs, allowing the use of environmentally friendly solvents, optimize analysts and equipment while providing quality analyses.en
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent520-524
dc.language.isoeng
dc.relation.ispartofCurrent Pharmaceutical Analysis
dc.sourceScopus
dc.subjectDegradation products
dc.subjectHPLC
dc.subjectRifaximin
dc.subjectStability
dc.subjectTablets
dc.subjectThin chromatography
dc.titleMethod indicative of stability for the determination of rifaximin and its degradation products by thin chromatographicen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionSchool of Pharmaceutical Sciences of Ribeirão Preto
dc.description.affiliationDepartment of Drugs and Pharmaceutics School of Pharmaceutical Sciences Univ Estadual Paulista- UNESP
dc.description.affiliationDepartment of Physics and Chemistry Center for Research in Natural and Synthetic Products School of Pharmaceutical Sciences of Ribeirão Preto
dc.description.affiliationUnespDepartment of Drugs and Pharmaceutics School of Pharmaceutical Sciences Univ Estadual Paulista- UNESP
dc.identifier.doi10.2174/1573412912666160801103712
dc.rights.accessRightsAcesso restrito
dc.identifier.scopus2-s2.0-85032884707
dc.relation.ispartofsjr0,224
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