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dc.contributor.authorZanchetta, Ariane [UNESP]
dc.contributor.authordos Santos, Antonio Carlos Freitas
dc.contributor.authorXimenes, Eduardo
dc.contributor.authorda Costa Carreira Nunes, Christiane [UNESP]
dc.contributor.authorBoscolo, Maurício [UNESP]
dc.contributor.authorGomes, Eleni [UNESP]
dc.contributor.authorLadisch, Michael R.
dc.date.accessioned2018-12-11T17:35:24Z
dc.date.available2018-12-11T17:35:24Z
dc.date.issued2018-03-01
dc.identifierhttp://dx.doi.org/10.1016/j.biortech.2017.12.061
dc.identifier.citationBioresource Technology, v. 252, p. 143-149.
dc.identifier.issn1873-2976
dc.identifier.issn0960-8524
dc.identifier.urihttp://hdl.handle.net/11449/179491
dc.description.abstractExtents of adsorption of cellulolytic enzymes on lignin, derived from sugarcane bagasse, were an inverse function of incubation temperature and varied with type of lignin extraction. At 45 °C, lignin derived from acid hydrolyzed liquid hot water pretreated bagasse completely adsorbed cellulolytic enzymes from Trichoderma reesei within 90 min. Lignin derived from enzyme hydrolyzed liquid hot water pretreated bagasse adsorbed only 60% of T. reesei endoglucanase, exoglucanase and β-glucosidase activities. β-Glucosidase from Aspergillus niger was not adsorbed. At 30 °C, adsorption of all of the enzymes was minimal and enzyme hydrolysis at 30 °C approached that at 45 °C after 168 h. Hence, temperature provided an approach to decrease loss of enzyme activity by reducing enzyme adsorption on lignin. This helps to explain why simultaneous saccharification and fermentation (SSF) and consolidated bioprocessing (CBP), both carried out at 30–32 °C, could offer viable options for mitigating lignin-derived inhibition effects.en
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipPurdue University
dc.format.extent143-149
dc.language.isoeng
dc.relation.ispartofBioresource Technology
dc.sourceScopus
dc.subjectAdsorption
dc.subjectCellulolytic enzymes
dc.subjectInhibition
dc.subjectLignin
dc.subjectSugarcane bagasse
dc.titleTemperature dependent cellulase adsorption on lignin from sugarcane bagasseen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionPurdue University
dc.description.affiliationSao Paulo State University-Unesp IBILCE São José do Rio Preto
dc.description.affiliationLaboratory of Renewable Resources Engineering Purdue University
dc.description.affiliationDepartment of Agricultural and Biological Engineering Purdue University
dc.description.affiliationUnespSao Paulo State University-Unesp IBILCE São José do Rio Preto
dc.identifier.doi10.1016/j.biortech.2017.12.061
dc.rights.accessRightsAcesso aberto
dc.identifier.scopus2-s2.0-85040074824
dc.identifier.file2-s2.0-85040074824.pdf
dc.relation.ispartofsjr2,029
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