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dc.contributor.authorde Barros, Patrícia Pimentel [UNESP]
dc.contributor.authorScorzoni, Liliana [UNESP]
dc.contributor.authorRibeiro, Felipe de Camargo [UNESP]
dc.contributor.authorFugisaki, Luciana Ruano de Oliveira [UNESP]
dc.contributor.authorFuchs, Beth Burgwyn
dc.contributor.authorMylonakis, Eleftherios
dc.contributor.authorJorge, Antonio Olavo Cardoso [UNESP]
dc.contributor.authorJunqueira, Juliana Campos [UNESP]
dc.contributor.authorRossoni, Rodnei Dennis [UNESP]
dc.date.accessioned2018-12-11T17:35:56Z
dc.date.available2018-12-11T17:35:56Z
dc.date.issued2018-04-01
dc.identifierhttp://dx.doi.org/10.1016/j.micpath.2018.02.019
dc.identifier.citationMicrobial Pathogenesis, v. 117, p. 80-87.
dc.identifier.issn1096-1208
dc.identifier.issn0882-4010
dc.identifier.urihttp://hdl.handle.net/11449/179588
dc.description.abstractThe objective of this study was to evaluate the influence of microbe-microbe interactions to identify a strain of Lactobacillus that could reduce the filamentation of Candida albicans ATCC 18804 using in vitro and in vivo models. Thus presenting a probiotic effect against the fungal pathogen. First, we analyzed the ability of 25 clinical isolates of Lactobacillus to reduce filamentation in C. albicans in vitro. We found that L. paracasei isolate 28.4 exhibited the greatest reduction of C. albicans hyphae (p = 0.0109). This reduction was confirmed by scanning electron microscopy analysis. The influence of C. albicans filamentation was found to be contributed through reduced gene expression of filament associated genes (TEC1 and UME6). In an in vivo study, prophylactic provisions with L. paracasei increased the survival of Caenorhabditis elegans worms infected with C. albicans (p = 0.0001) by 29%. Prolonged survival was accompanied by the prevention of cuticle rupture of 27% of the worms by filamentation of C. albicans, a phenotype that is characteristic of C. albicans killing of nematodes, compared to the control group. Lactobacillus paracasei isolate 28.4 reduced the filamentation of C. albicans in vitro by negatively regulating the TEC1 and UME6 genes that are essential for the production of hyphae. Prophylactic provision of Lactobacillus paracasei 28.4 protected C. elegans against candidiasis in vivo. L. paracasei 28.4 has the potential to be employed as an alternative method to control candidiasis.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent80-87
dc.language.isoeng
dc.relation.ispartofMicrobial Pathogenesis
dc.sourceScopus
dc.subjectCandida albicans
dc.subjectGene expression
dc.subjectLactobacillus
dc.subjectPathogenesis
dc.subjectProbiotics
dc.subjectYeasts
dc.titleLactobacillus paracasei 28.4 reduces in vitro hyphae formation of Candida albicans and prevents the filamentation in an experimental model of Caenorhabditis elegansen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionAlpert Medical School of Brown University
dc.description.affiliationDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology UNESP – Univ Estadual Paulista, Francisco Jose Longo 777, Sao Dimas
dc.description.affiliationDivision of Infectious Diseases Rhode Island Hospital Alpert Medical School of Brown University
dc.description.affiliationUnespDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology UNESP – Univ Estadual Paulista, Francisco Jose Longo 777, Sao Dimas
dc.identifier.doi10.1016/j.micpath.2018.02.019
dc.rights.accessRightsAcesso aberto
dc.description.sponsorshipIdFAPESP: 2013/25181-8
dc.description.sponsorshipIdFAPESP: 2014/12458-4
dc.description.sponsorshipIdFAPESP: 2015/09770-9
dc.identifier.scopus2-s2.0-85042053305
dc.identifier.file2-s2.0-85042053305.pdf
dc.relation.ispartofsjr0,751
dc.relation.ispartofsjr0,751
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