Hydrogen bioproduction with anaerobic bacteria consortium from brewery wastewater
MetadataShow full item record
Biohydrogen production is a cheap and clean way to obtain hydrogen gas. In subtropical countries such as Brazil the average temperatures of 27 °C can favor the hydrogen producing bacteria growth. A mixed culture was obtained from a subtropical sludge treating brewery wastewater and anaerobic batch reactors were fed with glucose, sucrose, fructose and xylose in low concentrations (2.0, 5.0 and 10.0 g L−1) at 37 °C, initial pH 5.5 and headspace with N2 (99%) to maintain the anaerobic conditions. The inoculum was a subtropical granulated sludge from UASB (Upflow Anaerobic Sludge Blanket) reactor treating brewery wastewater. The higher H2 yields were obtained in reactors operated with 2 and 5 g L−1 of fructose and they were 1.5 mol H2 mol−1 of fructose and 1.3 mol H2 mol−1 of sucrose, respectively. The volatile fatty acids (VFA) generated at the end of operation were, predominantly, butyric and acetic acid, indicating the favoring of the metabolic route of hydrogen generation by the consortium of anaerobic bacteria from the brewery wastewater. Biomolecular analyses revealed the predominance of hydrogen producing bacteria from Firmicutes phylum distributed in the families Streptococcaceae, Veillonellaceae and uncultured bacteria. These results confirm future applications of subtropical sludges with agroindustrial wastewaters containing low concentrations of sugars on hydrogen generation.
How to cite this document
Showing items related by title, author, creator and subject.
Pinho, Sheila Zambello de ; Oliveira, José Brás Barreto de ; Gazola, Rodrigo José Cristiano ; Mazotti, Adriano César ; Molero, Camila Schimite ; Mendes, Carolina Borghi ; Mello, Denise Fernandes de ; Marques, Emilia de Mendonça Rosa ; Talamoni, Jandira Liria Biscalquini ; Silva, José Humberto Dias da et al. (Coleção PROGRAD (UNESP), 2011) [Livro]
Pinho, Sheila Zambello de ; Oliveira, José Brás Barreto de ; Pontes, Sueli Rodrigues ; Almeida, Djanira Soares de Oliveira e ; Godoy, Kathya Maria Ayres de ; Rosa, Claudia de Souza ; Nunes, Julianus Araújo ; Salvador, Sérgio Azevedo ; David, Célia Maria ; Vilche Peña, Angel Fidel et al. (Coleção PROGRAD (UNESP), 2011) [Livro]
Pinho, Sheila Zambello de ; Spazziani, Maria de Lourdes ; Mendonça, Sueli Guadelupe de Lima ; Rubo, Elisabete Aparecida Andrello ; Villarreal, Dalva Maria de Oliveira ; Duarte, Camila ; Okamoto, Mary Yoko ; Souza, Thais R. ; Garms, Gilza Maria Zauhy ; Marin, Fátima Aparecida Dias Gomes et al. (Coleção PROGRAD (UNESP), 2012) [Livro]