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dc.contributor.authorCamacho, Sabrina A. [UNESP]
dc.contributor.authorSobral-Filho, Regivaldo Gomes
dc.contributor.authorAoki, Pedro Henrique B. [UNESP]
dc.contributor.authorConstantino, Carlos José L. [UNESP]
dc.contributor.authorBrolo, Alexandre G.
dc.date.accessioned2018-12-11T17:36:26Z
dc.date.available2018-12-11T17:36:26Z
dc.date.issued2018-03-23
dc.identifierhttp://dx.doi.org/10.1021/acssensors.7b00639
dc.identifier.citationACS Sensors, v. 3, n. 3, p. 587-594, 2018.
dc.identifier.issn2379-3694
dc.identifier.urihttp://hdl.handle.net/11449/179705
dc.description.abstractZika virus (ZIKV) was considered a public health emergency of international concern after the 2015 outbreak. Serological tests based on immunoassay platforms is one of the methods applied on the diagnosis of ZIKV and Dengue virus (DENV). However, the high limits of detection (LOD) and the cross-reactivity between ZIKV and DENV are still limitations in immunological tests. In order to tackle these issues, we have designed an immune-specific assay based on surface-enhanced Raman scattering (SERS) nanoprobes. Gold shell-isolated nanoparticles (Au-SHINs) were synthesized with 100 nm Au nanoparticles and 4 nm silica shell thickness coated with Nile Blue (Raman reporter). Then, the SERS nanoprobes were wrapped in a final silica shell and functionalized with monoclonal anti-ZIKV NS1 antibodies. Concentrations of ZIKV NS1 down to 10 ng/mL were probed free of cross-reactivity with DENV NS1 antigens.en
dc.format.extent587-594
dc.language.isoeng
dc.relation.ispartofACS Sensors
dc.sourceScopus
dc.subjectcross-reactivity
dc.subjectgold shell-isolated nanoparticles
dc.subjectRaman reporter
dc.subjectSERS nanoprobes
dc.subjectZika detection
dc.titleZika Immunoassay Based on Surface-Enhanced Raman Scattering Nanoprobesen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Victoria (UVic)
dc.description.affiliationSchool of Technology and Applied Sciences São Paulo State University (UNESP)
dc.description.affiliationDepartment of Chemistry Center for Advanced Materials and Related Technologies (CAMTEC) University of Victoria (UVic)
dc.description.affiliationSchool of Sciences Humanities and Languages São Paulo State University (UNESP)
dc.description.affiliationUnespSchool of Technology and Applied Sciences São Paulo State University (UNESP)
dc.description.affiliationUnespSchool of Sciences Humanities and Languages São Paulo State University (UNESP)
dc.identifier.doi10.1021/acssensors.7b00639
dc.rights.accessRightsAcesso restrito
dc.identifier.scopus2-s2.0-85044409845
dc.identifier.lattes7384168674539702
dc.identifier.orcid0000-0003-4701-6408
unesp.author.lattes7384168674539702[3]
unesp.author.orcid0000-0002-3162-0881[5]
unesp.author.orcid0000-0003-4701-6408[3]
dc.relation.ispartofsjr1,895
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