Biopolymer-based membranes associated with osteogenic growth peptide for guided bone regeneration
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Data
2018-03-14
Autores
Saska, Sybele [UNESP]
Pigossi, Suzane C [UNESP]
Oliveira, Guilherme J. P. L. [UNESP]
Teixeira, Lucas N.
Capela, Marisa V. [UNESP]
Gonçalves, Andreia [UNESP]
De Oliveira, Paulo T.
Messaddeq, Younès [UNESP]
Ribeiro, Sidney J. L. [UNESP]
Gaspar, Ana Maria Minarelli [UNESP]
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Barrier membranes for guided bone regeneration (GBR) mainly promote mechanical maintenance of bone defect space and induce osteopromotion. Additionally, biopolymer-based membranes may provide greater bioactivity and biocompatibility due to their similarity to extracellular matrix (ECM). In this study, biopolymers-based membranes from bacterial cellulose (BC) and collagen (COL) associated with osteogenic growth peptide (OGP(10-14)) were evaluated to determine in vitro osteoinductive potential in early osteogenesis; moreover, histological study was performed to evaluate the BC-COL OGP(10-14) membranes on bone healing after GBR in noncritical defects in rat femur. The results showed that the BC-COL and BC-COL OGP(10-14) membranes promoted cell proliferation and alkaline phosphatase activity in osteoblastic cell cultures. However, ECM mineralization was similar between cultures grown on BC OGP(10-14) and BC-COL OGP(10-14) membranes. In vivo results showed that all the membranes tested, including the peptide-free BC membrane, promoted better bone regeneration than control group. Furthermore, the BC-COL OGP(10-14) membranes induced higher radiographic density in the repaired bone than the other groups at 1, 4 and 16 weeks. Histomorphometric analyses revealed that the BC-COL OGP(10-14) induced higher percentage of bone tissue in the repaired area at 2 and 4 weeks than others membranes. In general, these biopolymer-based membranes might be potential candidates for bone regeneration applications.
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bacterial cellulose, barrier membrane, collagen
Como citar
Biomedical Materials (Bristol), v. 13, n. 3, 2018.