Eficácia da terapia fotodinâmica antimicrobiana associada a nistatina no tratamento de candidose oral em camundongos infectados com Candida albicans resistente a fluconazol
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Data
2018-11-26
Autores
Rimachi Hidalgo, Karem Janeth
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Universidade Estadual Paulista (Unesp)
Resumo
O objetivo do estudo foi avaliar a eficácia da terapia fotodinâmica antimicrobiana (aPDT) associada a Nistatina (NYS) no tratamento de candidose oral induzida em camundongos infectados com Candida albicans resistente a fluconazol. Foram utilizados 174 camundongos Swiss fêmeas com aproximadamente 5 semanas de vida. Os animais foram imunossuprimidos com predinisolona 100 mg/kg no 1º, 5º e 13 º dias de experimento. No 2º dia, os animais foram sedados com 0,1 mL de cloridrato de clorpromazina e uma suspensão de C. albicans 107 UFC/mL foi inoculada na língua dos mesmos. Do dia 7 ao 11 os tratamentos foram realizados. No grupo aPDT foi utilizado 200 mg/L de Photodithazine (PDZ) associado à luz LED de 50 J/cm2 (grupo P+L+); no grupo (P+L-) foi utilizado apenas com PDZ; o grupo (P-L+) recebeu só luz LED e nos animais do grupo NYS o medicamento foi aplicado uma vez ao dia. Além disso, foi avaliada a combinação de duas terapias: P+L+NYS e NYS+P+L+. Um grupo recebeu apenas inoculação de C. albicans (grupo P-L-) e outro grupo de animais saudáveis (grupo CNI). Após os tratamentos, foi realizada a recuperação de C. albicans por meio de swabs estéreis. Então diluições seriadas foram realizadas e plaqueadas em placas de Petri com SDA. Após 48 horas de incubação a 37o C as colônias foram quantificadas e o número de UFC/mL foi determinado. Os camundongos foram sacrificados 24 horas e 7 dias após os tratamentos. Os resultados demonstraram que a combinação das terapias promoveu redução de 2,6 log10 e 2,1 log10 para os grupos P+L+NYS+ e NYS+P+L+, respectivamente, 24 horas após os tratamentos. A análise macroscópica revelou remissão das lesões orais, e a análise histológica demonstrou expressiva redução da reação inflamatória no tecido que apresentou características histológicas de normalidade. Dessa forma, pode-se concluir que a combinação de terapias é uma alternativa para o tratamento de candidose induzida com cepas de C. albicans resistente ao fluconazol.
The aim of the present study was to evaluate of PDZ-mediated aPDT, as well as the association of this approach with antifungal nystatin, would be effective to treat oral candidosis in mice infected with fluconazole resistant C. albicans. Were used 174 female Swiss mice of 5 weeks-old-age approximately. The animals were immunosuppressed with predinisolone 100 mg/kg on the 1st, 5th and 13th days of the experiment. On day 2, the animals were sedated with 0.1 mL of chlorpromazine hydrochloride and a suspension of C. albicans 107 CFU/mL was inoculated into the tongue. From 7 to 11 the treatments were performed. In the aPDT group, was used 200 mg/L of Photodithazine (PDZ) associated with 50 J /cm2 LED light (P+L+ group). In the (P+L-) group was used only with PDZ; the group (P-L+) received only LED light and in the animals of the NYS group the drug was applied once a day. In addition, we evaluated the combination of two therapies: P+L+NYS and NYS+P+L+. One group received only inoculation of C. albicans (P-L- group) and another group of healthy animals (CNI group). After the treatments, recovery of C. albicans was performed by sterile swabs, then serial dilutions were performed and plated on Petri dishes with SDA. After 48 hours incubation at 37 °C the colonies were quantified, and the number of CFU/mL was determined. Mice were sacrificed 24 hours and 7 days after the treatments. The results showed that the combination of the therapies promoted reduction of 2.6 log10 and 2.1 log10 for the groups P+L+NYS+ and NYS+P+L+, respectively, 24 hours after the treatments. The macroscopic analysis revealed remission of oral lesions, and the histological analysis demonstrated an expressive reduction of the inflammatory reaction in the tissue that presented histological characteristics of normality. Thus, it can be concluded that the combination of therapies is an alternative for the treatment of candidosis induced with strains of C. albicans resistant to fluconazole.
The aim of the present study was to evaluate of PDZ-mediated aPDT, as well as the association of this approach with antifungal nystatin, would be effective to treat oral candidosis in mice infected with fluconazole resistant C. albicans. Were used 174 female Swiss mice of 5 weeks-old-age approximately. The animals were immunosuppressed with predinisolone 100 mg/kg on the 1st, 5th and 13th days of the experiment. On day 2, the animals were sedated with 0.1 mL of chlorpromazine hydrochloride and a suspension of C. albicans 107 CFU/mL was inoculated into the tongue. From 7 to 11 the treatments were performed. In the aPDT group, was used 200 mg/L of Photodithazine (PDZ) associated with 50 J /cm2 LED light (P+L+ group). In the (P+L-) group was used only with PDZ; the group (P-L+) received only LED light and in the animals of the NYS group the drug was applied once a day. In addition, we evaluated the combination of two therapies: P+L+NYS and NYS+P+L+. One group received only inoculation of C. albicans (P-L- group) and another group of healthy animals (CNI group). After the treatments, recovery of C. albicans was performed by sterile swabs, then serial dilutions were performed and plated on Petri dishes with SDA. After 48 hours incubation at 37 °C the colonies were quantified, and the number of CFU/mL was determined. Mice were sacrificed 24 hours and 7 days after the treatments. The results showed that the combination of the therapies promoted reduction of 2.6 log10 and 2.1 log10 for the groups P+L+NYS+ and NYS+P+L+, respectively, 24 hours after the treatments. The macroscopic analysis revealed remission of oral lesions, and the histological analysis demonstrated an expressive reduction of the inflammatory reaction in the tissue that presented histological characteristics of normality. Thus, it can be concluded that the combination of therapies is an alternative for the treatment of candidosis induced with strains of C. albicans resistant to fluconazole.
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Fotoquimioterapia, Candida albicans, Antifúngicos, Photochemotherapy, Candida albicans, Antifungals