Modulatory effects of verapamil in rifampicin activity against Mycobacterium tuberculosis
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Data
2019-02-01
Autores
Caleffi-Ferracioli, Katiany R.
Cardoso, Rosilene F.
Souza, Joao V. P. de
Murase, Leticia S.
Canezin, Pedro H.
Scodro, Regiane B. L.
Siqueira, Vera L. D.
Pavan, Fernando R. [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Future Medicine Ltd
Resumo
Aim: To evaluate modulatory effect of verapamil (VP) in rifampicin (RIF) activity and its effect in efflux pumps (EPs) transcript levels in Mycobacterium tuberculosis. Materials & methods: RIF and VP minimal inhibitory concentration, combinatory effect and detection of mutations were determined in 16 isolates. EPs transcript levels were determined in four isolates by real-time PCR after exposure to drugs. Results: VP showed good combinatory effect among RIF-resistant isolates. This effect was also observed in the relative transcript levels of EPs, mainly after 72h of exposure, depending on the EP gene, genotype and the resistance profile of the isolate. Conclusion: Additional regulatory mechanisms in the EP activities, as well as, interactions with other drug-specific resistance mechanisms need further investigation in M. tuberculosis.
Descrição
Palavras-chave
efflux pump inhibitor, gene regulation, multidrug transporters, Mycobacterium tuberculosis, real-time PCR, resistance, rifampicin, synergism, tuberculosis, verapamil
Como citar
Future Microbiology. London: Future Medicine Ltd, v. 14, n. 3, p. 185-194, 2019.