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dc.contributor.authorFernandes, Anderson
dc.contributor.authorSobrinho Scudeler, Patricia Elda [UNESP]
dc.contributor.authorDiniz, Debora [UNESP]
dc.contributor.authorForesti, Fausto
dc.contributor.authorde Oliveira Campos, Lucio Antonio
dc.contributor.authorLopes, Denilce Meneses
dc.date.accessioned2014-05-20T13:52:17Z
dc.date.available2014-05-20T13:52:17Z
dc.date.issued2011-11-01
dc.identifierhttp://dx.doi.org/10.1007/s13592-011-0082-0
dc.identifier.citationApidologie. Paris: Springer France, v. 42, n. 6, p. 743-748, 2011.
dc.identifier.issn0044-8435
dc.identifier.urihttp://hdl.handle.net/11449/18684
dc.description.abstractThe emergence of new molecular biology techniques has provided cytogenetics with tools which allow for the elucidation of questions that classical cytogenetics could not answer. Therefore, the present work standardizes a microdissection protocol for cytogenetic studies in bees. This methodology was first used in these insects and may contribute greatly to studies involving chromosomal rearrangements, heterochromatin composition, B chromosomes and others. For this study, the centromeric region of chromosomes in the stingless bee Tetragonisca fiebrigi was used for probe synthesis. The results demonstrated that the methodology used was efficient, presenting markings in the centromeric regions of several chromosomes. Hybridization in other sites indicates that the probe was able to detect regions that present homology with its sequence. This indicates that the technique is effective to study chromosomal evolution, genome organization and even the origin of B chromosomes.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent743-748
dc.language.isoeng
dc.publisherSpringer France
dc.relation.ispartofApidologie
dc.sourceWeb of Science
dc.subjectTetragoniscaen
dc.subjectHymenopteraen
dc.subjectcytogeneticsen
dc.subjectFISHen
dc.subjectmicrodissectionen
dc.titleMicrodissection: a tool for bee chromosome studiesen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer France
dc.contributor.institutionUniversidade Federal de Viçosa (UFV)
dc.contributor.institutionUniv Estado Mato Grosso
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual do Sudoeste da Bahia (UESB)
dc.description.affiliationUniversidade Federal de Viçosa (UFV), Dept Biol Geral, BR-36570000 Vicosa, MG, Brazil
dc.description.affiliationUniv Estado Mato Grosso, Dept Ciencias Biol, Tangara da Serra, Mato Grosso, Brazil
dc.description.affiliationUNESP, Dept Morfol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Sudoeste Bahia UESB, DCB, BR-45206190 Jequie, BA, Brazil
dc.description.affiliationUnespUNESP, Dept Morfol, BR-18618000 Botucatu, SP, Brazil
dc.identifier.doi10.1007/s13592-011-0082-0
dc.identifier.wosWOS:000297707400010
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
dc.identifier.lattes0804793944846367
unesp.author.lattes0804793944846367
unesp.author.orcid0000-0001-7209-4411[6]
dc.relation.ispartofjcr2.856
dc.relation.ispartofsjr1,220
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