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dc.contributor.authorMachado, Gustavo P.
dc.contributor.authorSilva, Rodrigo C.
dc.contributor.authorGuimarães, Felipe F. [UNESP]
dc.contributor.authorSalina, Anelise [UNESP]
dc.contributor.authorLangoni, Hélio [UNESP]
dc.date.accessioned2019-10-06T16:53:37Z
dc.date.available2019-10-06T16:53:37Z
dc.date.issued2018-07-01
dc.identifierhttp://dx.doi.org/10.1590/1678-5150-PVB-55141
dc.identifier.citationPesquisa Veterinaria Brasileira, v. 38, n. 7, p. 1358-1364, 2018.
dc.identifier.issn1678-5150
dc.identifier.issn0100-736X
dc.identifier.urihttp://hdl.handle.net/11449/189833
dc.description.abstractThis study evalueted the prevalence of Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in milk samples from 257 goats (513 half-udders) and ten bulk tanks, from ten dairy goat farms of São Paulo State, Brazil, by multiplex-PCR. The samples were screened by microbiological culture (gold-standard), and tested by different multiplex-PCR protocols for the detection of each bacterium. A total of 178 half-udders resulted positive by microbiological culture, with coagulase-negative staphylococci (70%), S. aureus (13.5%), S. intermedius (7.9%), and Enterobacteriaceae (4%) the prevalent pathogens. In other way, multiplex-PCR detected 173 pathogens in 151/523 (28.9%; CI95% 25.2-32.9%) milk samples 144/513 (28.1%) half-udders and 7/10 (70%) bulk tanks, with E. coli (86/162, 51.9%) and S. aureus (50/162, 30.9%) the prevalent ones in half-udders, and S. aureus (6/10, 60%) and E. coli (4/5, 36.4%) in bulk tanks. Multiplex-PCR showed a high performance for the detection of three bacteria at a time in mastitic goat milk direct from half-udders or bulk tanks. Thus, this multiplex-PCR protocol proved to be an adequate tool for the identification of the most common mastitis pathogens, independent of their phenotypic characteristics in the diagnosis of clinical mastitis in goats, allowing a continuous and better vigilance and monitoring the herd, being included in quality programs.en
dc.format.extent1358-1364
dc.language.isoeng
dc.relation.ispartofPesquisa Veterinaria Brasileira
dc.sourceScopus
dc.subjectBulk tanks
dc.subjectEscherichia coli
dc.subjectGoats
dc.subjectMastitis
dc.subjectMicrobiological culture
dc.subjectMilk
dc.subjectMultiplex-PCR
dc.subjectStaphylococcus aureus
dc.subjectStreptococcus agalactiae
dc.titleDetection of Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in Brazilian mastitic milk goats by multiplex-PCRen
dc.typeArtigo
dc.contributor.institutionMédico Veterinário
dc.contributor.institutionUniversidade Estadual do Norte do Paraná
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationMédico Veterinário, Rua Rodrigues Alves 227
dc.description.affiliationCentro de Ciências Agrárias Universidade Estadual do Norte do Paraná Campus Luiz Meneghel, Sala 88, Rodovia BR-369 Km 54
dc.description.affiliationDepartamento de Higiene Veterinária e Saúde Pública Universidade Estadual Paulista (Unesp), Rua Prof. Dr. Walter Maurício Correa s/n
dc.description.affiliationUnespDepartamento de Higiene Veterinária e Saúde Pública Universidade Estadual Paulista (Unesp), Rua Prof. Dr. Walter Maurício Correa s/n
dc.identifier.doi10.1590/1678-5150-PVB-55141
dc.identifier.scieloS0100-736X2018000701358
dc.rights.accessRightsAcesso aberto
dc.identifier.scopus2-s2.0-85055321529
dc.identifier.fileS0100-736X2018000701358.pdf
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