Show simple item record

dc.contributor.authorGuilger-Casagrande, Mariana
dc.contributor.authorGermano-Costa, Tais
dc.contributor.authorPasquoto-Stigliani, Tatiane
dc.contributor.authorFraceto, Leonardo Fernandes
dc.contributor.authorLima, Renata de
dc.date.accessioned2019-10-10T19:09:04Z
dc.date.available2019-10-10T19:09:04Z
dc.date.issued2019-10-04
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11449/190700
dc.description.abstractBiogenic synthesis of silver nanoparticles employing fungi offers advantages, including the formation of a capping from fungal biomolecules, which provides stability and can contribute to biological activity. In this work, silver nanoparticles were synthesized using Trichoderma harzianum cultivated with (AgNP-TS) and without enzymatic stimulation (AgNP-T) by the cell wall of Sclerotinia sclerotiorum. The nanoparticles were evaluated for the control of S. sclerotiorum. The specific activity of the T. harzianum hydrolytic enzymes were determined in the filtrates and nanoparticles. Cytotoxicity and genotoxicity were also evaluated. Both the nanoparticles exhibited inhibitory activity towards S. sclerotiorum, with no new sclerotia development, however AgNP-TS was more effective against mycelial growth. Both the filtrates and the nanoparticles showed specific enzymatic activity. Low levels of cytotoxicity and genotoxicity were observed. This study opens perspectives for further exploration of fungal biogenic nanoparticles, indicating their use for the control of S. sclerotiorum and other agricultural pests.pt
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.language.isopor
dc.publisherNature
dc.relation.ispartofScientific Reportspt
dc.subjectSilver nanoparticlespt
dc.subjectAgricultural pestspt
dc.titleBiosynthesis of silver nanoparticles employing Trichoderma harzianum with enzymatic stimulation for the control of Sclerotinia sclerotiorumpt
dc.typeArtigo
dc.description.versionVersão final do editorpt
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUniversidade Estadual Paulista Julio de Mesquita Filho
dc.description.affiliationLaboratory for Evaluation of the Bioactivity and Toxicology of Nanomaterials, University of Sorocaba (UNISO)
dc.identifier.doihttps://doi.org/10.1038/s41598-019-50871-0
dc.rights.accessRightsAcesso aberto
dc.description.sponsorshipId2017/21004-5
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Ciência e Tecnologia, Sorocabapt
dc.identifier.lattes4741480538883395
dc.identifier.orcidhttps://orcid.org/0000-0002-2827-2038
Localize o texto completo

Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record