Ação de aditivos moleculares em dispersões de vesículas e filmes de Langmuir de moléculas anfifílicas
Carregando...
Arquivos
Data
2019-10-29
Autores
Lemos, Monique
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
A utilização de carreadores não virais de fármacos, de baixo custo comercial, requer estudo das propriedades físico-químicas e caracterização desses carreadores. Uma opção para carreador não viral são vesículas de lipídios sintéticos ou naturais, como os catiônicos brometo e cloreto de dioctadecildimetilamônio (DODAB e DODAC) e o zwitterionico dipalmitoilfosfatidilcolina (DPPC) respectivamente. O presente estudo apresentam os efeitos dos sais NaCl e NaBr, e da glicose nas propriedades térmicas e estruturais desses lipídios organizados em monocamadas (filmes de Langmuir) ou em bicamadas (vesículas). Foi também investigada ação do copolímero Plurônico F127, como helper, na solubilização da molécula anticancerígena doxorrubicina nas vesículas investigadas, em soro fisiológico, glicosado e caseiro como solvente. A ação desses aditivos nas vesículas e monocamadas é investigada por calorimetria diferencial de varredura, espalhamento dinâmico da luz, espalhamento eletroforético da luz, espectrofotômetro-UV e medidas em cuba de Langmuir. Dentre os resultados obtidos destacam-se a formação e caracterização de vesículas em soro e na presença do copolímero F127 e DOX.
The use of low cost non-viral carriers of pharmaceutical drugs in medicine requires studies on the physico-chemical properties and characterization of such carriers. An option for non-viral carriers are vesicles formed with synthetic or natural amphiphiles, such as the cationic dioctadecyldimethylammonium bromide and chloride (DODAB and DODAC) and the zwitterionic 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), respectively. The present study introduces the effect of NaCl, NaBr and glucose on the thermal and structural properties of these lipids organized as monolayers (Langmuir films) or bilayers (vesicles). The action of the micelle-forming triblock copolymer Pluronic F127, as a helper was also investigated, on the solubilization of the anticancer molecule doxorubicin on the vesicles in saline, glucoside and saline/glucoside mixtures. The action of these additives for vesicles and monolayers were investigated by differential scanning calorimetry (DSC), dynamic light scattering (DLS), dynamic electrophoretic light scattering (DELS), UV-Vis spectrophotometry, and Lagmuir Films techniques. Among the results obtained, it is highlighted the formation and characterization of vesicles and monolayers under physiological conditions of the solvent, and in presence of F127 and DOX.
The use of low cost non-viral carriers of pharmaceutical drugs in medicine requires studies on the physico-chemical properties and characterization of such carriers. An option for non-viral carriers are vesicles formed with synthetic or natural amphiphiles, such as the cationic dioctadecyldimethylammonium bromide and chloride (DODAB and DODAC) and the zwitterionic 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), respectively. The present study introduces the effect of NaCl, NaBr and glucose on the thermal and structural properties of these lipids organized as monolayers (Langmuir films) or bilayers (vesicles). The action of the micelle-forming triblock copolymer Pluronic F127, as a helper was also investigated, on the solubilization of the anticancer molecule doxorubicin on the vesicles in saline, glucoside and saline/glucoside mixtures. The action of these additives for vesicles and monolayers were investigated by differential scanning calorimetry (DSC), dynamic light scattering (DLS), dynamic electrophoretic light scattering (DELS), UV-Vis spectrophotometry, and Lagmuir Films techniques. Among the results obtained, it is highlighted the formation and characterization of vesicles and monolayers under physiological conditions of the solvent, and in presence of F127 and DOX.
Descrição
Palavras-chave
Vesículas anfifílicas, DODAB, DODAC, DPPC, Plurônico F127, Contra íon, Glicose, Amphiphilic vesicles, Pluronic F127, Counterion, Glucose