Highly Sensitive Neurotransmitters Analysis at Platinum-Ultramicroelectrodes Arrays
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Data
2012-05-01
Autores
Moraes, Fernando C.
Cesarino, Ivana
Coelho, Dyovani
Pedrosa, Valber A. [UNESP]
Machado, Sergio A. S.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Wiley-v C H Verlag Gmbh
Resumo
An easy way to determine norepinephrine (NE) in biological fluid using a platinum ultramicroelectrode array (Pt-UMEAs) is described. Issues related to UME electrode surface treatment and characterizations are also addressed. At optimized experimental conditions the dynamic concentration range was 1.0 to 10.0 mu mol?L-1 with a detection limit of 40.5 nmol?L-1. The repeatability of current responses for injections of 5 mu mol?L-1 NE was evaluated to be 4.0?% (n=10). This approach obtained excellent sensitivity, a reliable calibration profile and stable electrochemical response for norepinephrine detection. The content of NE in urine samples without any preconcentration, purification, or pretreatment step, was successfully analyzed by the standard addition method using the Pt-UMEAs.
Descrição
Palavras-chave
Platinum ultramicroelectrode array, Norepinephrine, Biological fluids
Como citar
Electroanalysis. Weinheim: Wiley-v C H Verlag Gmbh, v. 24, n. 5, p. 1115-1120, 2012.