Structural and biological characterization of two novel peptides from the venom of the neotropical social wasp Agelaia pallipes pallipes

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Data

2004-07-01

Autores

Mendes, M. A.
de Souza, B. M.
Marques, M. R.
Palma, Mario Sergio [UNESP]

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Editor

Elsevier B.V.

Resumo

The venom of the neotropical social wasp Agelaia pallipes pallipes was fractionated by RP-HPLC resulting in the elution of seven fractions; the last two were re-fractionated under RP-HPLC by using isocratic elution conditions and the purity of the fractions were confirmed by using ESI-MS analysis. Both fractions are constituted of peptide components, which were sequenced by Edman degradation chemistry, resulting in the following sequences:Protonectin I-L-G-T-I-L-G-L-L-K-G-L-NH2Agelaia-MP I-N-W-L-K-L-G-K-A-I-I-D-A-L-NH2Both peptides are manually synthesized on solid-phase and functionally characterized by using Wistar rats cells. Protonectin is a non-hemolytic chemotactic peptide for polymorphonucleated leukocytes (PMNL), presenting some mast cell degranulating activity and potent antimicrobial action both against Gram-positive and Gram-negative bacteria. Agelaia-MP was characterized as a hemolytic mast cell degranulator toxin, presenting a poor antimicrobial action and no chemotaxis for PMNL. (C) 2004 Elsevier Ltd. All rights reserved.

Descrição

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social wasp, chemotactic peptide, mastoparan, hemolysis, antimicrobial peptide

Como citar

Toxicon. Oxford: Pergamon-Elsevier B.V., v. 44, n. 1, p. 67-74, 2004.