DETECTION OF THE PEPTIDE P17-1 (HIV) BY SURFACE ENHANCE RAMAN SCATTERING (SERS)
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Data
2019-01-01
Autores
Carneiro, Leandro B. [UNESP]
Moraes, Marli L.
Ribeiro, Sidney J. L. [UNESP]
Brolo, Alexandre G.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Soc Brasileira Quimica
Resumo
A bioanalytical method for HIV detection based on surface-enhanced Raman scattering (SERS) is described. The assay targeted the peptide p17-1. This peptide sequence is present in the protein p17, a structural protein essential in the life cycle of the human immunodeficiency retrovirus (HIV). The antibody anti-p17 was immobilized in a planar gold surface using self-assembled techniques. This planar platform was used to capture the target analyte (p17-1 peptide). The peptide detection was carried out using specially designed SERS probes, consisting of gold nanoparticles coated with a Raman reporter molecule (Nile Blue A) and functionalized with anti-p17. This sandwich assay led to the successful detection of the peptide p17-1 at 40.8 ng/mL levels. The sandwich assay described here demonstrated that the SERS technique can rival more common fluorescence methods, such as ELISA, in the detection HIV-type virus.
Descrição
Palavras-chave
HIV, SERS, peptide p17-1, SERS probes, SERS Immunoassay
Como citar
Quimica Nova. Sao Paulo: Soc Brasileira Quimica, v. 42, n. 9, p. 1038-1043, 2019.