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dc.contributor.authorBaptista-Saidemberg, Nicoli B. [UNESP]
dc.contributor.authorSaidemberg, Daniel Menezes
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.identifier.citationJournal of Proteomics. Amsterdam: Elsevier B.V., v. 74, n. 10, p. 2123-2137, 2011.
dc.description.abstractThe wasp Agelaia pallipes pallipes is one of the most aggressive species from the neotropical region, causing many stinging accidents every year, characterized by severe envenoming reactions. The identification of peptides is important for understanding the envenoming process; however, the tiny amount of venom produced by these insects makes this task a challenge, using classical analytical approaches. Thus, the venom was previously fractionated, and the sequences were obtained through the use of electrospray ionization with a tridimensional ion-trap and time-of-flight mass analysis under CID conditions. This approach permitted the sequence assignment of nine peptides. The presence of type -d and -w ions generated from the fragmentation of the side chains was used to resolve I/L ambiguity. The distinction between K and Q residues was achieved through esterification of the alpha- and epsilon-amino groups in the peptides, followed by mass spectrometry analysis. Six of these peptides were short, linear and polycationic, while the three other peptides presented a single disulfide bridge. The use of reduction and alkylation protocols, followed by ESI-IT-TOF/MS analysis under CID conditions, permitted easy sequencing of the three peptides presenting this post-translational modification. These peptides presented activity related to mast cell degranulation, hemolysis, or even the chemotaxis of leukocytes. (C) 2011 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Proteomics
dc.sourceWeb of Science
dc.subjectPolycationic peptidesen
dc.subjectWasp venomen
dc.titleProfiling the peptidome of the venom from the social wasp Agelaia pallipes pallipesen
dcterms.rightsHolderElsevier B.V.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationSão Paulo State Univ UNESP, CEIS Dept Biol, Inst Biosci, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, CEIS Dept Biol, Inst Biosci, BR-13506900 Rio Claro, SP, Brazil
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdFAPESP: 06/57122-7
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt
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