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dc.contributor.authordos Santos, Lucilene Delazari [UNESP]
dc.contributor.authorSantos, Keity Souza
dc.contributor.authorAparecido Pinto, Jose Roberto [UNESP]
dc.contributor.authorDias, Nathalia Baptista [UNESP]
dc.contributor.authorde Souza, Bibiana Monson [UNESP]
dc.contributor.authordos Santos, Manse Fonseca
dc.contributor.authorPerales, Jonas
dc.contributor.authorDomont, Gilberto Barbosa
dc.contributor.authorCastro, Fabio Morato
dc.contributor.authorKalil, Jorge Elias
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.date.accessioned2014-05-20T13:55:29Z
dc.date.available2014-05-20T13:55:29Z
dc.date.issued2010-08-01
dc.identifierhttp://dx.doi.org/10.1021/pr1000829
dc.identifier.citationJournal of Proteome Research. Washington: Amer Chemical Soc, v. 9, n. 8, p. 3867-3877, 2010.
dc.identifier.issn1535-3893
dc.identifier.urihttp://hdl.handle.net/11449/19852
dc.description.abstractThe study reported here is a classical bottom-up proteomic approach where proteins from wasp venom were extracted and separated by 2-DE; the individual protein spots were proteolytically digested and subsequently identified by using tandem mass spectrometry and database query with the protein search engine MASCOT. Eighty-four venom proteins belonging to 12 different molecular functions were identified. These proteins were classified into three groups; the first is constituted of typical venom proteins: antigens-5, hyaluronidases, phospholipases, heat shock proteins, metalloproteinases, metalloproteinase-desintegrin like proteins, serine proteinases, proteinase inhibitors, vascular endothelial growth factor-related protein, arginine kinases, Sol i-II and -II like proteins, alpha-glucosidase, and superoxide dismutases. The second contained proteins structurally related to the muscles that involves the venom reservoir. The third group, associated with the housekeeping of cells from venom glands, was composed of enzymes, membrane proteins of different types, and transcriptional factors. The composition of P. paulista venom permits us to hypothesize about a general envenoming mechanism based on five actions: (i) diffusion of venom through the tissues and to the blood, (ii) tissue, (iii) hemolysis, (iv) inflammation, and (v) allergy-played by antigen-5, PLA1, hyaluronidase, HSP 60, HSP 90, and arginine kinases.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent3867-3877
dc.language.isoeng
dc.publisherAmer Chemical Soc
dc.relation.ispartofJournal of Proteome Research
dc.sourceWeb of Science
dc.subjectsocial wasp venomen
dc.subjectPolybia paulistaen
dc.subjectHymenopteraen
dc.subjectallergyen
dc.subjectimmunoreactivityen
dc.subject2-D electrophoresisen
dc.subjectmass spectrometryen
dc.subjectenvenoming mechanismen
dc.titleProfiling the Proteome of the Venom from the Social Wasp Polybia paulista: A Clue to Understand the Envenoming Mechanismen
dc.typeArtigo
dcterms.licensehttp://pubs.acs.org/page/copyright/journals/faqs.html
dcterms.rightsHolderAmer Chemical Soc
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionFundação Oswaldo Cruz Mato Grosso do Sul (FIOCRUZ-MS)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.description.affiliationUniv São Paulo State UNESP, Ctr Study Social Insects, Dept Biol, Inst Biosci Rio Claro, Rio Claro, SP, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Dept Biochem, BR-21941 Rio de Janeiro, Brazil
dc.description.affiliationFiocruz MS, Dept Physiol & Pharmacodynam, Rio de Janeiro, Brazil
dc.description.affiliationDiscipline Allergy & Immunol InCor HC FMUSP, São Paulo, Brazil
dc.description.affiliationUnespUniv São Paulo State UNESP, Ctr Study Social Insects, Dept Biol, Inst Biosci Rio Claro, Rio Claro, SP, Brazil
dc.identifier.doi10.1021/pr1000829
dc.identifier.wosWOS:000280583700010
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdFAPESP: 05/00982-1
dc.description.sponsorshipIdFAPESP: 06/57122-7
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt
dc.identifier.lattes2901888624506535
unesp.author.lattes2901888624506535
unesp.author.orcid0000-0002-7363-8211[11]
unesp.author.orcid0000-0001-5832-1825[1]
unesp.author.orcid0000-0002-6211-5773[9]
dc.relation.ispartofjcr3.950
dc.relation.ispartofsjr1,818
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