Proteomic View of the Venom from the Fire Ant Solenopsis invicta Buren

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Data

2012-09-01

Autores

dos Santos Pinto, Jose R. A. [UNESP]
Fox, Eduardo G. P.
Saidemberg, Daniel M. [UNESP]
Santos, Lucilene D. [UNESP]
da Silva Menegasso, Anally R. [UNESP]
Costa-Manso, Eliude
Machado, Ednildo A.
Bueno, Odair C. [UNESP]
Palma, Mario Sergio [UNESP]

Título da Revista

ISSN da Revista

Título de Volume

Editor

Amer Chemical Soc

Resumo

Fire ants are well-known by their aggressive stinging behavior, causing many stinging incidents of medical importance. The limited availability of fire ant venom for scientific and clinical uses has restricted, up to now, the knowledge about the biochemistry, immunology, and pharmacology of these venoms. For this study, S. invicta venom was obtained commercially and used for proteomic characterization. For this purpose, the combination of gel-based and gel-free proteomic strategies was used to assign the proteomic profile of the venom from the fire ant S. invicta. This experimental approach permitted the identification of 46 proteins, which were organized into four different groups according to their potential role in fire ant venom: true venom components, housekeeping proteins, body muscle proteins, and proteins involved in chemical communication. The active venom components that may not present toxic roles were classified into three subgroups according to their potential functions: self-venom protection, colony asepsis, and chemical communication. Meanwhile, the proteins classified as true toxins, based on their functions after being injected into the victims' bodies by the fire ants, were classified in five other subgroups: proteins influencing the homeostasis of the victims, neurotoxins, proteins that promote venom diffusion, proteins that cause tissue damage/inflammation, and allergens.

Descrição

Palavras-chave

Hymenoptera, allergen, toxin, proteomic analysis, mass spectrometry, shotgun proteomics, envenoming mechanism

Como citar

Journal of Proteome Research. Washington: Amer Chemical Soc, v. 11, n. 9, p. 4643-4653, 2012.