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dc.contributor.authorAbdalla, Fábio Camargo [UNESP]
dc.contributor.authorCruz-Landim, Carminda da [UNESP]
dc.date.accessioned2014-05-20T13:55:37Z
dc.date.available2014-05-20T13:55:37Z
dc.date.issued2004-01-01
dc.identifierhttp://dx.doi.org/10.1267/ahc.37.65
dc.identifier.citationActa Histochemica Et Cytochemica. Kyoto: Japan Soc Histochem Cytochem, v. 37, n. 2, p. 65-71, 2004.
dc.identifier.issn0044-5991
dc.identifier.urihttp://hdl.handle.net/11449/19911
dc.description.abstractDufour glands of Apis mellifera and Melipona bicolor were studied under light and transmission electron microscopy, using the cytochemical techniques of mercury bromophenol blue for protein detection, imidazole-buffered osmium tetroxide selective staining of unsaturated lipids, lanthanum nitrate for intercellular junction identification and zinc-iodide-osmium tetroxide for cytoplasmic endomembrane visualization. The results in both species corroborated the lipid nature of the gland secretion and showed in A. mellifera the poverty of the synthetic machinery in the worker gland cells in comparison with the queen, as expected by previous biochemical analyses. The pathway of the exogenous compounds of the secretion is intracellular, since substances can penetrate the cell folds and intercellular junctions, but their access to the, gland lumen is barred by the apical intercellular junctions.en
dc.format.extent65-71
dc.language.isoeng
dc.publisherJapan Soc Histochem Cytochem
dc.relation.ispartofActa Histochemica et Cytochemica
dc.sourceWeb of Science
dc.subjectdufour glandpt
dc.subjectApis melliferapt
dc.subjectMelipona bicolorpt
dc.subjectcytochemistrypt
dc.titleA comparative cytochemical study of the dufour gland in the eusocial bee Apis mellifera Linne, 1758 and Melipona bicolor Lepeletier, 1836en
dc.typeArtigo
dcterms.licensehttps://www.jstage.jst.go.jp/pub/html/AY04S120_en.html
dcterms.rightsHolderJapan Soc Histochem Cytochem
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUNESP, Inst Biociencias, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.identifier.doi10.1267/ahc.37.65
dc.identifier.wosWOS:000220573200001
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Rio Claropt
dc.identifier.fileWOS000220573200001.pdf
dc.relation.ispartofjcr0.872
dc.relation.ispartofsjr0,446
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