Radioautographic study of the seasonal distribution of leukocytes in turtles Phrynops hilarii (Chelonia Chelidae)
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Data
2008-12-01
Autores
Pitol, Dimitrius Leonardo [UNESP]
Mardegan Issa, Joao Paulo
Caetano, Flávio Henrique [UNESP]
Lunardi, Laurelucia Orive
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Editor
Pergamon-Elsevier B.V. Ltd
Resumo
The aim of this study was to present a morphological description of the leukocytes of Phrynops hilarii turtles according to the seasonal distribution of these cells and to show their replacement in the blood circulation using a radioautographic method. Five animals of both sexes weighing 600-1200 g were used. The animal's blood was aspirated, smeared on glass slides, and stained with the Romanowsky stain, and 500 cells of each animal were counted during each season. The results obtained were analyzed statistically by analysis of variance followed by the Bonferrom test (NCSS), with the level of significance set at p < 0.05. The radioautographic analysis of turtle blood exposed to 1000 mu Ci of H-3-thymidine and developed after 30 days showed a large number of silver grains incorporated into the cells, except for basophils, with cell renewal occurring every seven days. Quantitative data demonstrated a seasonal influence on the distribution of some leukocyte types, with the following p values: heterophils (p = 0.0007), basophils (p = 0.0002), monocytes (p = 0.0016), eosinophils (p = 0.0073). However, using this statistical method, it was not possible to detect a significant difference related to seasonal influence on lymphocytes (p = 0. 16295) or thrombocytes (p = 0. 1046). Using this experimental animal model, a seasonal influence on the distribution of some leukocyte types was observed, and the radioautographic method revealed a cell renewal system occurring every seven days, except for basophils. (C) 2008 Elsevier Ltd. All rights reserved.
Descrição
Palavras-chave
Turtle, Leukocytes, Seasonal, Radioautography, Phrynops hilarii
Como citar
Micron. Oxford: Pergamon-Elsevier B.V. Ltd, v. 39, n. 8, p. 1381-1386, 2008.