Cross-reactive IgE antibody responses to tropomyosins from Ascaris lumbricoides and cockroach
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Data
2008-04-01
Autores
Santos, Ana Beatriz R.
Rocha, Gutemberg M.
Oliver, Constance
Ferriani, Virginia P. L.
Lima, Rodrigo C.
Palma, Mario Sergio [UNESP]
Sales, Valdria S. F.
Aalberse, Rob C.
Chapman, Martin D.
Arruda, L. Karla
Título da Revista
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Título de Volume
Editor
Mosby-elsevier
Resumo
Background: Evidence indicates that infection with Ascaris lumbricoides may promote development of allergy and asthma.Objective: To study the role of tropomyosin, a pan-allergen in invertebrates, in IgE responses to A lumbricoides.Methods: Recombinant A lumbricoides and Periplaneta americana tropomyosins were expressed in Pichia pastoris. Levels of IgE to tropomyosins from A lumbricoides and P americana were determined by chimeric ELISA in sera from 119 children living in a parasite-endemic area and 112 patients with cockroach allergy from the allergy clinics. Presence of tropomyosin in A lumbricoides larvae at L3 stage was evaluated by immunofluorescence using mAb IA6, directed against mite tropomyosin. Molecular modeling of P americana and A lumbricoides tropomyosins was performed by using the MODELLER program.Results: A lumbricoides tropomyosin showed 69% to 98% sequence identity to tropomyosins from other invertebrates. The predicted structure of A lumbricoides tropomyosin was similar to that of P americana tropomyosin and showed the characteristic coiled-coil structure. Strong correlation was found for IgE antibodies to tropomyosins from A lumbricoides and P americana in sera from children living in a parasite-endemic area and from patients with cockroach allergy. Larvae of A lumbricoides reacted strongly with mAb IA6.Conclusion: Tropomyosin induces IgE responses in A lumbricoides-infected children and in patients allergic to cockroach.
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tropomyosin, Ascaris lumbricoides, periplaneta americana, IgE cross-reactivity, allergy, parasites, asthma
Como citar
Journal of Allergy and Clinical Immunology. New York: Mosby-elsevier, v. 121, n. 4, p. 1040-1046, 2008.