Chronic alcoholism associated with diabetes induced apoptosis in the corpus cavernosum of rats

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2020-01-01

Autores

Schimming, Bruno Cesar [UNESP]
Cirino, Mucio Luiz de Assis
Neto, Fermino Sanches Lizarte
Novais, Paulo Cezar
de Carvalho, Camila Albuquerque Melo
Tirapelli, Daniela Pretti da Cunha
Molina, Carlos Augusto Fernandes
Tirapelli, Luis Fernando
Junior, Silvio Tucci

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Resumo

Purpose: To evaluate the effects of alcohol exposure and diabetes on apoptotic process in the corpus cavernosum. Methods: Forty eight male Wistar rats were divided into four groups: control, diabetic, alcoholic and diabetic-alcoholic. Samples of the corpus cavernosum were prepared to study protein expression of apoptotic genes (Caspases-3 and 9) by immunohistochemistry and Real-Time PCR. Results: The immunoreactivity of Caspases-3 and-9 was diffuse and higher in the treated groups though there was no significant difference between the experimental groups, only when compared with the control group. An increase was observed in the gene expression of Caspases-9 in the diabetic and ethanol-diabetic groups when compared with control and ethanol groups. Conclusions: The association of these factors (ethanol and diabetes) probably can affect the apoptosis mechanism in lesions of the cavernous tissue in the rat penis. Both gene and protein expression of Caspase-9 in diabetic and ethanol-diabetic groups suggest the involvement of the apoptosis cascade from this study model.

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Caspases, Diabetes Mellitus, Ethanol, Rats

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Acta Cirurgica Brasileira, v. 35, n. 8, p. 1-7, 2020.