A fast-multiclass method for the determination of 21 endocrine disruptors in human urine by using vortex-assisted dispersive liquid-liquid microextraction (VADLLME) and LC-MS/MS
Nenhuma Miniatura disponível
Data
2020-10-01
Autores
Bocato, Mariana Zuccherato
Cesila, Cibele Aparecida
Lataro, Beatriz Favero
de Oliveira, Anderson Rodrigo Moraes [UNESP]
Campíglia, Andres Dobal
Barbosa Jr, Fernando
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Simplicity, speed, and reduced cost are essential demands for routine analysis in human biomonitoring studies. Moreover, the availability of higher volumes of human specimens is becoming more restrictive due to ethical controls and to the costs associated with sample transportation and storage. Thus, analytical methods requiring much lower sample volumes associated with simultaneous detection capability (multiclass analysis) are with a very high claim. In this sense, the present approach aimed at the development of a method for preconcentration and simultaneous determination of four classes of endocrine disruptors (seven bisphenols, seven parabens, five benzophenones, and two antimicrobials) in the urine. The approach is based on vortex-assisted dispersive liquid-liquid microextraction (VADLLME) and high-performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). After optimization of the significant parameters of VADLLME extraction, the proposed procedure showed to be simple, fast, sensitive, requiring only 1.0 mL of urine, 400 μL of organic solvents with a total stirring time of 20 s. Moreover, a variation of inter-day and between-day runs were lower than 10.0% and 11.0%, respectively. Finally, the proposed method was successfully applied to the analysis of 50 urine samples of Brazilian pregnant women to establish reference ranges.
Descrição
Palavras-chave
Endocrine disruptors, Human urine, Liquid chromatography-tandem mass spectrometry, Multiclass analysis, Vortex-assisted dispersive liquid-liquid microextraction
Como citar
Environmental Research, v. 189.