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dc.contributor.authorMantellatto, Aline Meira Bonfim [UNESP]
dc.contributor.authorGonzález, Susana
dc.contributor.authorDuarte, José Maurício Barbanti [UNESP]
dc.date.accessioned2020-12-12T02:38:50Z
dc.date.available2020-12-12T02:38:50Z
dc.date.issued2020-01-01
dc.identifierhttp://dx.doi.org/10.1590/1678-4685-gmb-2019-0008
dc.identifier.citationGenetics and Molecular Biology, v. 43, n. 2, 2020.
dc.identifier.issn1678-4685
dc.identifier.issn1415-4757
dc.identifier.urihttp://hdl.handle.net/11449/201674
dc.description.abstractNatural history museum collections constitute an invaluable patrimony of biological diversity for analysing the taxa distribution and evolution. However, it is very common to discover taxonomic misidentification in museum collections based on incorrect data. The aim of this research was to identify brocket deer species (Mazama genus) using molecular markers. We collected 199 samples, performed DNA extraction and species identification using a specific mito-chondrial marker based on a fragment of cytochrome b (Cytb) for Neotropical deer. We achieved the amplification and sequencing of 77 specimens and verified that 26% of the skulls were wrongly identified. Moreover, in the museum collections 57% of the specimens were only identified as Mazama sp, and we were able to identify them by molecular methods to the species level. Our findings clearly demonstrate the importance of integrating molecular analyses to identify Mazama species, since using only external morphology can result in a high probability of errors. We recommend the selection of non-convergent morphological characters, which together with the use of DNA collected from museum specimens should contribute to more accurate taxonomic identifications.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.language.isoeng
dc.relation.ispartofGenetics and Molecular Biology
dc.sourceScopus
dc.subjectCytochrome b
dc.subjectDeer
dc.subjectDNA
dc.subjectMammal
dc.subjectMorphological taxonomy
dc.titleMolecular identification of Mazama species (Cervidae: Artiodactyla) from natural history collectionsen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionLaboratório de Ecologia e Conservação Marinha
dc.contributor.institutionInstituto de Investigaciones Biológicas Clemente Estable
dc.description.affiliationUniversidade Estadual Paulista “Júlio de Mesquota Filho” (UNESP) Faculdade de Ciências Agrárias e Veterinárias Núcleo de Pesquisa e Conservação de Cervídeos
dc.description.affiliationUniversidade Federal do Sul da Bahia Centro de Formação em Ciências Ambientais Laboratório de Ecologia e Conservação Marinha, Campus Sosígenes Costa
dc.description.affiliationInstituto de Investigaciones Biológicas Clemente Estable Departamento de Biodiversidad y Genética
dc.description.affiliationUnespUniversidade Estadual Paulista “Júlio de Mesquota Filho” (UNESP) Faculdade de Ciências Agrárias e Veterinárias Núcleo de Pesquisa e Conservação de Cervídeos
dc.identifier.doi10.1590/1678-4685-gmb-2019-0008
dc.identifier.scieloS1415-47572020000400201
dc.rights.accessRightsAcesso aberto
dc.description.sponsorshipIdFAPESP: 2013/05944-7
dc.identifier.scopus2-s2.0-85082960293
dc.identifier.fileS1415-47572020000400201.pdf
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