Morphological and molecular analysis of apoptosis in the corpus cavernosum of rats submitted to a chronic alcoholism model1
Carregando...
Data
2020-01-01
Autores
Meirelles, Rogério José de Azevedo
Neto, Fermino Sanches Lizarte
Cirino, Mucio Luiz de Assis
Novais, Paulo Cezar
Gula, Isabella Stracieri
Silva, Jairo Pinheiro da
Tazzima, Maria de Fátima Galli Sorita
Fazan, Valéria Paula Sassoli
Durand, Marina Toledo
Tirapelli, Daniela Pretti da Cunha [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Purpose: To evaluate the effect of chronic alcoholism on morphometry and apoptosis mechanism and correlate with miRNA-21 expression in the corpus cavernosum of rats. Methods: Twenty-four rats were divided into two experimental groups: Control (C) and Alcoholic group (A). After two weeks of an adaptive phase, rats from group A received only ethanol solution (20%) during 7 weeks. The morphometric and caspase-3 immunohistochemistry analysis were performed in the corpus cavernosum. The miRNA-21 expression was analyzed in blood and cavernous tissue. Results: Chronic ethanol consumption decreased cavernosal smooth muscle area of alcoholic rats. The protein expression of caspase 3 in the corpus cavernosum was higher in A compared to the C group. There was no difference in the expression of miRNA-21 in serum and cavernous tissue between the groups. Conclusion: Chronic ethanol consumption reduced smooth muscle area and increased caspase 3 in the corpus cavernosum of rats, without altered serum and cavernosal miR-21 gene expression.
Descrição
Palavras-chave
Apoptosis, Erectile Dysfunction, Ethanol, MicroRNAs, Rats
Como citar
Acta Cirurgica Brasileira, v. 35, n. 3, p. 1-8, 2020.