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dc.contributor.authorKassuya, Roberto Mikio
dc.contributor.authorRadai, Joyce Alencar Santos
dc.contributor.authorMacorini, Luis Fernando Benitez
dc.contributor.authorNunes, Viktor Krejci
dc.contributor.authorSalvador, Marcos José
dc.contributor.authorLeite, Patricia Regina Terço
dc.contributor.authorOliveira, Rodrigo Juliano
dc.contributor.authorCroda, Júlio
dc.contributor.authorArena, Arielle Cristina [UNESP]
dc.contributor.authorKassuya, Cândida Aparecida Leite
dc.description.abstractInformation on the health benefits of ethanolic extracts obtained from Blutaparon portulacoides stem (EEBP) hasn´t been consistently described in the literature until the present moment. This study investigated the antimycobacterial, anti-inflammatory and toxicological effects of EEBP in models of inflammation/infection, as well as its chemical composition. Chemical analysis of EEBP by electrospray ionization–mass spectrometry/HPLC–MS/MS identified 3,5,3′-Trihydroxy-4′-methoxy-6,7-methylenedioxy-flavone, gomphrenol, ferulic, vanillic, and caffeic acids. The minimum inhibitory concentration of EEBP and isoniazid in the presence of Mycobacterium tuberculosis was 123.4 and 0.030 µg/ml, respectively. EEBP oral administration (p.o.) (300–1000 mg/kg) or dexamethasone subcutaneous injection (s.c.) (1 mg/kg) significantly inhibited leukocytes and proteins resulting from carrageenan-induced pleurisy in Swiss mice. In the BCG-induced pleurisy model, the oral treatments performed once a day for 7 days, with EEBP (30 and 100 mg/kg) and isoniazid (25 mg/kg), inhibited the increase in plasmatic IL-1β levels and in pleural exudate from C57BL-6 mice, and reduced M. tuberculosis growth in organs (colony forming units assays). EEBP (30–300 mg/kg, p.o.) and dexamethasone (1 mg/s.c.) significantly prevented carrageenan-induced oedema and mechanical hyperalgesia in Swiss mice. The treatments (once a day for 22 days) with EEBP (30 mg/kg, p.o.) and dexamethasone (1 mg/s.c.) substantially inhibited oedema and mechanical- and cold-hyperalgesia at 11, 16 and 22 days after the administration of Freund's Complete Adjuvant in C57bL6 mice. No evidence of physio-pathologic was observed in Wistar rats acutely treated with EEBP (2000 mg/kg, p.o.). This study confirms the anti-inflammatory and antibiotic properties of EEBP, opening possibilities for the development of safe new drugs with dual anti-inflammatory/antimycobacterial activities which could be favorable from a pharmacoeconomic perspective.en
dc.subjectBlutaparon portulacoides
dc.subjectMycobacterium tuberculosis
dc.titleBlutaparon portulacoides ethanolic extract reduced IL-1β and inflammatory parameters induced by the Mycobacterium complex and carrageenan in miceen
dc.contributor.institutionFederal University of Grande Dourados
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionFederal University of Mato Grosso Do Sul
dc.contributor.institutionYale University School of Public Health
dc.contributor.institutionOswaldo Cruz Foundation
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationFaculty of Health Sciences Federal University of Grande Dourados
dc.description.affiliationDepartment of Plant Biology PPG BTPB and PPG BCE Institute of Biology University of Campinas (UNICAMP), Campinas
dc.description.affiliationSchool of Medicine Federal University of Mato Grosso Do Sul
dc.description.affiliationDepartment of Epidemiology of Microbial Diseases Yale University School of Public Health
dc.description.affiliationOswaldo Cruz Foundation
dc.description.affiliationDepartment of Structural and Functional Biology Institute of Biosciences of Botucatu Universidade Estadual Paulista (UNESP) - Botucatu
dc.description.affiliationUnespDepartment of Structural and Functional Biology Institute of Biosciences of Botucatu Universidade Estadual Paulista (UNESP) - Botucatu
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