Show simple item record

dc.contributor.authorGarcia Simoes, Maria Lucia
dc.contributor.authorTauk-Tornisielo, Samia Maria [UNESP]
dc.contributor.authorTapia Tapia, Daniel Mario
dc.date.accessioned2013-09-30T18:48:35Z
dc.date.accessioned2014-05-20T13:57:45Z
dc.date.available2013-09-30T18:48:35Z
dc.date.available2014-05-20T13:57:45Z
dc.date.issued2009-11-16
dc.identifierhttp://www.academicjournals.org/AJB/abstracts/abs2009/16Nov/Simoes%20et%20al.htm
dc.identifier.citationAfrican Journal of Biotechnology. Victoria Island: Academic Journals, v. 8, n. 22, p. 6317-6326, 2009.
dc.identifier.issn1684-5315
dc.identifier.urihttp://hdl.handle.net/11449/20581
dc.description.abstractThe objective of this research was to investigate xylanase production by filamentous fungi (Trichoderma viride) to determine the best cultivation conditions in the process, aiming toward optimization of enzyme production. The best temperature, as well as the best carbon source, for biomass production was determined through an automated turbidimetric method (Bioscreen-C). The enzyme activity of this fungus was separately evaluated in two solid substrates (wheat and soybean bran) and in Vogel medium, pure and by adding other carbon sources. Temperature effects, cultivation time, and spore concentrations were also tested. The best temperature and carbon source for enzyme and biomass production was 25 C and sorbitol, respectively. Maximum xylanase activity was achieved when the fungus was cultivated in wheat bran along with sorbitol (1%, w/v), using a spore concentration of 2 x 10(6) spores. mL(-1), pH 5.0, for 144 h cultivation. The study demonstrated not only the importance of the nature of the substrate in obtaining a system resistant to catabolic repression, but also the importance of the culture conditions for biosynthesis of this enzyme. T. viride showed a high potential for xylanase production under the conditions presented in these assays.en
dc.description.sponsorshipCentro de Estudos Ambientais (CEA) at UNESP
dc.description.sponsorshipPostgraduate Course in Biological Sciences, Applied Microbiology, at the Rio Claro, SP, Campus of UNESP
dc.format.extent6317-6326
dc.language.isoeng
dc.publisherAcademic Journals
dc.relation.ispartofAfrican Journal of Biotechnology
dc.sourceWeb of Science
dc.subjectTrichoderma virideen
dc.subjectxylanase activityen
dc.subjectenzyme optimizationen
dc.titleScreening of culture condition for xylanase production by filamentous fungien
dc.typeArtigo
dcterms.licensehttp://www.academicjournals.org/Creative%20Common%20Attribution%20License.htm
dcterms.rightsHolderAcademic Journals
dc.contributor.institutionUniv Estadual Sudoeste Bahia
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUniv Estadual Sudoeste Bahia, BR-45050020 Vitoria da Conquista, BA, Brazil
dc.description.affiliationUniv Estadual Paulista, Ctr Estudos Ambientais, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Ctr Estudos Ambientais, BR-13506900 Rio Claro, SP, Brazil
dc.identifier.wosWOS:000273718900049
dc.rights.accessRightsAcesso aberto
dc.identifier.fileWOS000273718900049.pdf
Localize o texto completo

Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record